Title of Document: OXYGEN MEASUREMENT DURING CELL CULTURE: FROM MULTIWELL-PLATES TO MICROFLUIDIC DEVICES Peter Chung Thomas, Doctor of Philosophy 2011 Directed By: Professor Srinivasa R. Raghavan, Department of Chemical and Biomolecular Engineering Oxygen is an important regulator of normal cell behavior. Proper supply of oxygen is required to maintain ATP production, while perturbation of oxyg...

This paper presents data characterizing the ability to measure metabolic activity at a tissue interface using a Clark-type oxygen concentration sensor consisting of a microfabricated thinfilm electrode matrix overlaid with a solid-state proton conductive matrix and encapsulated in a bio-inert polytetrafluoroethylene film. HL-1 atrial myocytes were cultured onto the sensor surface. Cyclic voltam...

BACKGROUND Dissolved oxygen tension (DOT) is an important parameter for evaluating a bioprocess. Conventional means to measure DOT in shake flasks using fixed Clark-type electrodes immersed in the bulk liquid are problematic, because they inherently alter the hydrodynamics of the systems. Other approaches to measure DOT that apply fluorescing sensor spots fixed at the inside wall of a shake fla...