Extraction of soybean seed proteins for two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and mass spectrometry analysis is challenging and inconsistent. In this study, we compared four different protein extraction/solubilization methods-urea, thiourea/urea, phenol, and a modified trichloroacetic acid (TCA)/acetone-to determine their efficacy in separating soybean seed proteins by 2D...

ABSTRACT Infection of peanut (Arachis hypogaea) seed by Aspergillus flavus and A. parasiticus is a serious problem that can result in aflatoxin contamination in the seed. Breeding resistant cultivars would be an effective approach to reduce aflatoxin accumulation. The objective of this study was to investigate the expression of the pathogenesis-related (PR) protein beta-1,3-glucanase and the is...

This study was performed to investigate the effect of storage conditions on seed germination performance of faba bean (cv. Shami). For this purpose, a three factors factorial experiment was conducted in laboratory of Seed Science and Technology, Faculty of Agriculture, Yasouj University, in 2013-2014, based on CRD design with five replications.  The experimental factors were storage temperature...

Wild progenitors of rice (Oryza) are an invaluable resource for restoring genetic diversity and incorporating useful traits back into cultivars. Studies were conducted to characterize the biochemical changes, including SDS-PAGE banding pattern of storage proteins in seeds of six wild species (Oryza alta, O. grandiglumis, O. meridionalis, O. nivara, O. officinalis and O. rhizomatis) of rice stor...

Storage conditions of oil seeds before industrial extraction might influence the quality of the crude oil. The aim of present study was to clarify the storage able potency of high and low oleic cultivars of sunflower based on oil and fatty acid changes of seeds and their viability. The seed samples of four sunflower cultivars (two high oleic and two low oleic) were stored in three different tem...

SSPs (Seed Storage Proteins) of the 11S type have been preliminary characterized in the seeds of the argan tree, an endemic species from Morocco. Protein extracts from mature seeds were prepared by using different solutions in order to assess the solubility of the major protein forms. SSPs of the 11S type were classified as albumins according to the further SDS-PAGE analysis of these extracts. ...

somaclonal variation is one of the possible sources of variation in plant breeding. to evaluate the usefulness of somaclonal variation for creating variation in iranian cultivars of wheat, proteins, enzymes and chromosome number of regenerated and seed-produced plants of wheat (alamout, hyrmand and maroon) were studied. plantlets obtained from the immature embryos and seed culture were collecte...