نتایج جستجو برای: patch clamp techniques
تعداد نتایج: 678546 فیلتر نتایج به سال:
The chloride (Cl-) channel cystic fibrosis transmembrane conductance regulator (CFTR) is defective in cystic fibrosis (CF), and mutation of its encoding gene leads to various defects such as retention of the misfolded protein in the endoplasmic reticulum, reduced stability at the plasma membrane, abnormal channel gating with low open probability, and thermal instability, which leads to inactiva...
The patch clamp technique was first used by Neher and Sakmann (1976) to resolve currents through single acetylcholine-activated channels in cell-attached patches of membrane of frog skeletal muscle. The method they used (described by Neher, Sakmann & Steinbach, 1978) and subsequent refinements (Hamill, Marty, Neher, Sakmann & Sigworth, 1981) have led to techniques for high resolution recording ...
ive and are unsuitable for impaling electrodes. Methods for modelling the kinetics and permeation of Much of our knowledge of ion-transport mechanisms ion channels using single-channel recordings are prein plant cell membranes comes from experiments sented. How the ionic currents underlying an action using voltage-clamp. This technique allows the measpotential (AP) can be identified using the A...
Patch-clamp recording is a powerful approach to monitoring membrane electrical activity with high temporal resolution. However, the spatial resolution of patch-clamp recording in a distributed structure such as a neuron or a brain slice is limited by the fact that each electrode records from just one point, making recording from more than a very small number of points impractical. An approach t...
Planar patch-clamp is a two-dimensional variation of traditional patch-clamp. By contrast to classical glass micropipette, the seal quality of silicon patch-clamp chips (i.e. seal resistance and seal success rate) have remained poor due to the planar geometry and the nature of the substrate and thus partially obliterate the advantages related to planar patch-clamp. The characterization of physi...
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