AGGAATCCCATG-30 and CTX-M-R2, 50-GCCGTCTAAGGCGATAAAC-30, were used to amplify the entire allele (876 bp) for sequencing. This revealed that S. enterica serovar Senftenberg strain 9-4 produced CTX-M-3, which was first identified in Poland. Southern hybridization using the whole blaCTX-M gene as a probe showed that the gene was located on a plasmid of < 38 kb in size. Attempts to transfer this pl...

Sonlu bir Z_n grubunun yönlendirilmemiş P(Z_n ) power grafı, köşelerinin kümesi olan bağlantılı graftır ve burada iki köşe komşudur gerek yeter şart u≠v ⊆ veya dir. 〖(Z〗_n,+) grubuna karşılık gelen Yönlendirilmemiş G=P(Z_n grafının Hyper-Wiener, SK, 〖SK〗_1, 〖SK〗_2 Harary indeksleri sırasıyla, 1/2 ∑_({u,v}⊆V(G))▒〖d(u,v)〗+1/2 ∑_({u,v}⊆V(G))▒〖d^2 (u,v) 〗 ∑_(uv∈E(G))▒〖d(u)+d(v)〗, ∑_(uv∈E(G))▒〖d(u)....

The gene coding for opine dehydrogenase from Arthrobacter sp. strain 1C was cloned onto plasmid pBluescript KS(-), and the nucleotide sequence of the 1,077-bp open reading frame consisting of 359 codons was identified as the odh gene. Transformed Escherichia coli cells overproduced NAD+-dependent opine dehydrogenase under control of the promoter of the lac gene on pBluescript KS(-).

Ligation of a DNA restriction fragment into a plasmid vector, followed by isolation of a recombinant plasmid, is a fundamental procedure in molecular biology. Standard protocols require at least two days to go from unligated DNA to the isolation of sufficient quantities of recombinant plasmid for restriction enzyme analysis or DNA sequencing (1,3). The two most timeconsuming steps involve the s...

k=1 q1−s k r s k log θk (S1) where p(s) = Pr(s1, . . . , sK|u,v, s) = ∏K k=1 Pr(sk|u,v, s). pk = P (sk = 1|u,v) is given by eq. (2). qk = P (sk = 1|u,v, s = 0) and rk = P (sk = 1|u,v, s = 1) are given by eq. (7). Note that the last equation uses the fact that Ep(s)(sk) = q 1−s k r s k. The third term Ep(s)[s log(1− ∏K k=1 θ 1(sk=1) k )] is not tractable; variational methods can be used, as desc...

Endothelial cells (ECs) regulate the barrier function of blood vessels. Here we show that basal and angiopoietin-1 (Ang-1)-regulated control of EC permeability is mediated by 2 different functional states of sphingosine kinase-1 (SK-1). Mice depleted of SK-1 have increased vascular leakiness, whereas mice transgenic for SK-1 in ECs show attenuation of leakiness. Furthermore, Ang-1 rapidly and t...