BACKGROUND AND OBJECTIVES We earlier reported elevated chemokine ligand-2 (CCL2) in Indian amyotrophic lateral sclerosis (ALS) patients. We now analysed chemokine receptor-2 (CCR2), the receptor of CCL2, in these ALS patients. METHODS Indian sporadic ALS patients (n=50) were included on the basis of El Escorial criteria. Percentage (%) of CCR2 expressing peripheral blood mononuclear cells (PB...

Use of the CD28/B7 costimulatory signal for T-cell activation was analyzed in granulocyte colony-stimulating factor (G-CSF) mobilized peripheral blood mononuclear cells (G-PBMCs) and in peripheral blood mononuclear cells obtained before administration of G-CSF (preG-PBMCs). CTLA4Ig inhibition of OKT3-stimulated proliferation was significantly lower in G-PBMCs compared with preG-PBMCs (39.9% +/-...

BACKGROUND Behçet disease (BD) is a relapsing inflammatory disease with increased production of inflammatory cytokines in peripheral blood mononuclear cells (PBMCs); however, the underlying molecular mechanisms are not well known. OBJECTIVE To analyze whether the differential expression of transcription factors is involved in the increased tumor necrosis factor (TNF)-α and interleukin (IL)-6 ...

background: similar to inflammatory cells, peripheral blood mononuclear cells (pbmcs) can also infiltrate in to kidney and urinary tracts and subsequently excreted by urine. in this study we determined the viability rate and response to phytohemagglutinin-a (pha) of human pbmcs in normal urine. methods: a number of 1×106 ficoll-hypaque isolated pbmcs were dispensed in 1 ml normal urine and 6 mo...

Background: Vitamin D insufficiency and deficiency can be associated with adverse effects on fetus and pregnancy outcomes. This study aimed at evaluating the effect of 1,25VitD3 on specific transcription factor and markers of Tregs and Th17 cells in the PBMCs of women with URPL as a case group and the PBMCs of healthy women as a control group. Methods: Samples from 20 non-pregnant patients with...

Inflamed synovium of rheumatoid arthritis (RA) has been associated with a T helper (Th)1 cytokine profile but the blood situation remains to be clarified. We studied the differential IFN-gamma producing activity of peripheral blood mononuclear cells (PBMCs) from RA patients (RA-PBMCs) and from healthy controls (H-PBMCs) in response to IL-12 and IL-18. RA-PBMCs had a decreased IFN-gamma producti...

BACKGROUND Eicosanoids mediate initiation and resolution of inflammation. Our aim was evaluating the effects of training, exercise and docosahexaenoic (DHA) supplementation on plasma eicosanoids levels and peripheral blood mononuclear cells (PBMCs) eicosanoids production. METHODS Fifteen male footballers were distributed to placebo and experimental groups. Experimental group consumed DHA-enri...

Sirtuin 1 (SIRT1) has been described to modify immune responses by modulation of gene transcription. As transcriptional reprogramming is the molecular substrate trained immunity, a de facto innate memory, we investigated role SIRT1 in induction immunity. We identified various genetic single nucleotide polymorphisms affecting and adaptive cytokine production human peripheral blood mononuclear ce...

We unraveled the expression profiles of coding-noncoding RNAs in intervertebral disc degeneration (IDD). However, it remains elusive regarding miR-155 peripheral blood mononuclear cells (PBMCs) and local extracellular space IDD. The study aimed for investigating PBMCs, miRNAs (ex-miRNAs) human nucleus pulposus (NP) tissues, morphological changes cell death IDD process. Here, we harvested NP sam...