GC-rich DNA regions were PCR-amplified with Taq DNA polymerase using either the canonical set of deoxynucleoside triphosphates or mixtures in which the dCTP had been partially or completely replaced by its N4-methylated analog, N4-methyl-2'-deoxycytidine 5'-triphosphate (N4me-dCTP). In the case of a particularly GC-rich region (78.9% GC), the PCR mixtures containing N4me-dCTP produced the expec...

there has been a great increase in the consumption of various food additives in recent years. the purpose of this study was to identify the incidence of sensitization to food additives by using skin prick test in patients with allergy and to determine the concordance rate between positive skin tests and oral challenge in hypersensitivity to additives. this cross-sectional study included 125 (fe...

The hallowed notion that oxidative damage causes aging and that vitamins might preserve our youth is now in doubt

We studied the influence of using different powders and several additives on the shaping process and critical current density of sintered bulk YBa2Cu30x' It was found that all additives used facilitated the shaping process. Silver and most organic additives influenced the critical current density only little, Zinc reduced it to zero, but an ethylenebisstearoylamide compound doubled the critical...

The role of food additives in chronic urticaria (CU) is still under investigation. In this study, we aimed to explore the association between food additives and CU by using the basophil activation test (BAT). The BAT using 15 common food additives was performed for 15 patients with CU who had a history of recurrent urticarial aggravation following intake of various foods without a definite food...

the chemical composition, in situ  rumen degradability coefficients of dry matter (dm) and crude protein (cp), in vitro gas production and in vivo  digestibility were evaluated for olive cake (oc) silage treated (dm basis) with different additives as follows: (1) untreated oc; (2) oc, 8% molasses and 0.4% formic acid and (3) oc, 8% molasses, 0.4% formic acid and 0.5% urea. after addition of the...

pyruvate kinase is a key enzyme in glycolytic pathway that catalyzes the transphosphorylation between phosphoenolpyruvate and adp to yield atp and pyruvate. geobacillus stearothermophillus has a stable pyruvate kinase with determined crystal structure that composed of four separate domains. given that limited proteolysis experiments can be successfully used to probe conformational features of p...

In this study, flat sheet asymmetric polyphenylsulfone (PPSU) ultrafiltration membranes with enhanced antifouling properties were prepared with a non-solvent induced phase separation (NIPS) method through compound additives containing a polymeric pore-forming agent, a small molecular non-solvent and a surfactant. The formation processes of the porous asymmetric membranes with different kinds of...

Optical and FTIR spectroscopy was employed to study the properties of 80GeS2-20Ga2S3-CsCl chalcohalide glasses with CsCl additives in a temperature range of 77-293 K. It is shown that CsCl content results in the shift of fundamental absorption edge in the visible region. Vibrational bands in FTIR spectra of (80GeS2-20Ga2S3)100 - х (СsCl) x (x = 5, 10, and 15) are identified near 2500 cm(-1), 37...