نتایج جستجو برای: pcr elisa
تعداد نتایج: 212744 فیلتر نتایج به سال:
abstract beet yellows virus (byv), the type member of the genus closterovirus and one of the most economically important yellowing viruses, causes serious losses in sugarbeet. in order to study infection and distribution of beet yellows virus during summer 2007, about 530 samples were collected from beet’s fields in razavi khorasan province (fariman, torbate jam, ghoochan, chenaran, torbate hey...
background: visceral leishmaniasis (vl) is caused by leishmania infantum in mediterranean basin and is an endemic disease in some parts of iran. canines are the main reservoirs of vl in most of the endemic areas. different serological methods have been introduced for diagnosis of canine visceral leishmaniasis (cvl). objective: in this survey a fucose-mannose ligand (fml) elisa, using native l. ...
zucchini yellow mosaic virus (zymv) is a potyvirus with a worldwide distribution. this virus causes serious economic losses in iran in many cucurbits. during 2002-2003, sam-ples were collected from squash fields in tehran province. five isolates (z1, z2, z3, z4 and z5) were inoculated on 27 species of cucurbitaceae, chenopodiaceae, amarantha-ceae,solanacae, leguminosae and ranunculaceae. chenop...
A total of 355 cows were sampled (serum, n = 315; faeces, n = 355; milk, n = 209) from dairy farms located in the Punjab state of India. Faeces and serum/milk samples were screened by acid fast staining and "indigenous ELISA," respectively. IS900 PCR was used to screen faeces and milk samples. Bio-load of MAP in dairy cows was 36.9, 15.6, 16.3, and 14.4%, using microscopy, serum ELISA, milk ELI...
BACKGROUND Feline immunodeficiency virus (FIV) infection is an important cause of disease of cats worldwide. Initial screening is commonly performed by commercially available point-of-care (POC) ELISA tests. Confirmatory testing for positive POC test results is recommended. Polymerase chain reaction (PCR) tests for FIV are commonly used additional testing methods; however, reported measures of ...
Background and objectives: Cutaneous leishmaniasis is endemic in most areas of Iran, and the diagnosis of its species is essential for controlling the disease. Leishmania major is the causative agent for cutaneous leishmaniasis in humans. Molecular methods are generally more sensitive than microscopic methods. The present study aimed to use a polymerase chain reaction-enzyme linked immunosorben...
Labeling of food allergens in accordance with legal regulations is important to protect the health allergic consumers. The requirements for detecting foods involve adequate specificity and sensitivity identify very small amounts target complex matrices processed foods. In this work, one hundred commercial samples were analyzed walnut detection using three different methods: a sandwich enzyme-li...
background: the ability to sensitively detect vibrio cholera with pcr-elisa method represents a considerable advancement over alternative more time-consuming methods for detection of this pathogen. the aim of this research is to evaluate the suitability of a pcr-enzyme-linked immunosorbent assay for sensitive and rapid detection of v. cholera o1. methods: the 398-bp sequence of a gene that code...
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