To obtain a better understanding of metabolically active microbial communities, we tested a molecular ecological approach using poly(A) tailing of environmental 16S rRNA, followed by full-length complementary DNA (cDNA) synthesis and sequencing to eliminate potential biases caused by mismatching of polymerase chain reaction (PCR) primer sequences. The RNA pool tested was extracted from marine s...

Thalassemias are hereditary anemia syndromes occurring due to erroneous producing of globin chain of hemoglobin. Thalassemia syndromes are named according to the type of the affected globin chain. The most common types of thalassemia are alpha thalassemia and beta thalassemia. In α-thalassemia, α-globin chain production is either by decreased or completely disappeared. The gene encoding the α-g...

A minute of silence. Kary Banks Mullis has died (08/07/2019). was an American Biochemist and Doctor Chemistry who developed the Polymerase Chain Reaction (PCR) in 1985, considered one most important legacies history Humanity, along with description DNA (Watson, Crick Franklin) theory Relativity (Einstein). Currently, I consider anyone does not know PCR by to be illiterate, since it is a molecul...

In this paper, we show an integrated complementary metal oxide semiconductor (CMOS)-based microchip system with capillary array electrophoresis (CAE) for the detection of bacterial pathogen amplified by polymerase chain reaction (PCR). In order to demonstrate the efficacy of PCR reaction for the heat-labile toxin producing enterotoxigenic Escherichia coli (E. coli), which causes cholera-like di...

Purpose: Telomerase is considered a molecular marker for malignancy. The aim of this study was to determine telomer-ase activity (TA) as a prognostic factor at diagnosis and as a marker for minimal residual disease during therapy and follow up in nonmetastatic Ewing family of tumors (EFT). Patients and Methods: Primary tumor specimens and 97 peripheral blood (PBL) samples from 31 EFT patients w...

PURPOSE Although the etiology of breast cancer is multifactorial, oxidative stress plays an important role in carcinogenesis. In this study, manganese superoxide dismutase (MnSOD) gene polymorphism and activity were evaluated in benign and breast cancer tissue. METHODS One hundred and one females were enrolled in this study, 65 who were histopathologically diagnosed with breast cancer and 46 ...

BACKGROUND Numerous in vitro reports suggest that Low Level Laser Therapy (LLLT) affects cellular processes by biostimulation, however most of them emphasize on using visible light lasers which have low penetration. The aim of this study was to determine the effect of infrared laser light (which is more useful in clinic because of its higher penetration) on secretion of Fibroblast Growth Factor...

In this paper, we investigated the activation energies of the aggregation−disaggregation self-oscillation induced by the Belousov-Zhabotinsky (BZ) reaction by utilizing the nonthermoresponsive polymer chain in a wide temperature range. This is because the conventional type self-oscillating polymer chain, with thermoresponsive poly(Nisopropylacrylamide) (poly(NIPAAm) main-chain covalently bonded...