background and objective: the outer membrane protein w ( ompw ) of vibrio cholerae is involved in stimulating the im- mune response via induction of protective immunity. it also plays an important role in bacterial pathogenesis by increasing the adaptability of pathogenic strains. in this study we aimed to clone v. cholerae ompw gene in the strain x-33 of pichia pastoris. materials and methods:...

The application of bacteriophages biocontrol requires the formulation genetically distinct in a phage cocktail. Random Amplified Polymorphic DNA (RAPD) - PCR is considered cheap, reproducible, and readily applicable tool detecting diversity compared to other molecular techniques such as whole-genome sequencing. We used this study RAPD-PCR technique assess genetic 28 infecting Pseudomonas aerugi...

The polymerase chain reaction (PCR) has made possible the rapid isolation and amplification of specific DNA segments, which can then be used for a wide range of applications (1). Direct sequencing of PCR products has been described, using assymetric PCR, which requires purification of the PCR product, or inclusion of 10% DMSO in the sequencing reaction (2, 3, 4). However, these methods of seque...

Analyzing EGFR mutations and detecting ALK gene fusion are indispensable when planning to treat pulmonary adenocarcinoma. Malignant pleural effusion (MPE) is a devastating complication of lung cancer and sometimes the only source for mutation analysis. The percentage of tumor cells in the pleural effusion may be low; therefore, mutant enrichment is required for a successful analysis. The EGFR m...

optimization of the condition for pcr-directed sequencing of microsatellites poly adenine (a) length polymorphisms is more difficult and sensitive compared with other common sequences. replication slippage may occur for polymerase enzyme during microsatellite amplification and direct sequencing of these pcr products will be challenging for heterozygote samples. so, the aim of this study is to i...