Data was searched against a Swiss-Prot® complete human database with a 1% FDR criteria using Percolator. Analysis and interpretation of DIA data were performed using Biognosys Spectronaut 7. A library was built from the combined search outputs of Proteome Discoverer 1.4 using a high confidence peptide filter and fragment filter settings of 300 to 1800 m/z with fragments greater than y3 or b3. M...

Tandem mass spectrometry is commonly used to identify peptides (and thereby proteins) that are present in complex mixtures. Peptide identification from tandem mass spectra is partially automated, but still requires human curation to resolve "borderline" peptide-spectrum matches (PSMs). SILVER is web-based software that assists manual curation of tandem mass spectra, using a recently developed i...

PMF is one of the major methods for protein identification using the MS technology. It is faster and cheaper than MS/MS. Although PMF does not differentiate trypsin-digested peptides of identical mass, which makes it less informative than MS/MS, current computational methods for PMF have the potential to improve its detection accuracy by better use of the information content in PMF spectra. We ...

Loss of heterozygosity and allelic imbalance in tumors are usually detected by either radioactive labeling of PCR products with subsequent scoring of autoradiographs or by a semi-quantitative fluorescence-based protocol. Polymorphic microsatellite loci are the most common marker type used in these studies. Even though no consensus exists as to how to evaluate such data, results are often compar...

this study was conducted to evaluate the role of swimming on mechanic of lung in healthy individual and patients with asthma. a total 76 girls who took part in the course of regular swimming session three day per week for eight weeks enrolled in this study. all of them completed isaac written questionnaire and individual who was suspected of asthma or other atopic diseases was referred to aller...

With the expansion of metro network, large passenger flow in peak hours has brought great challenges to operation. While upgrading security inspection, traffic efficiency passengers stations also been affected. Considering public and inspection efficiency, using method credit rating establish a differentiated mode become development direction intelligent future. It is necessary study evaluation...

osteoporosis is a major public health problem in iran. bone densitometry is used to diagnose osteopenia and osteoporosis and if necessary, prevent bone fractures, especially that of femoral neck. bone density is related to several factors including race, age, sex, environmental factors and nutrition. no comprehensive study has been performed in iran, yet. among the 10 to 75 year-old population ...