Vibrio harveyi siphophage 1 (VHS1) is a tailed phage with an icosahedral head of approximately 66 nm in diameter and an unornamented, flexible tail of approximately 153 nm in length. When Vibrio harveyi 1114GL is lysogenized with VHS1, its virulence for the black tiger shrimp (Penaeus monodon) increases by more than 100 times, and this coincides with production of a toxin(s) associated with shr...

The population of Vibrios in diseased Penaeus monodon collected from culture ponds situated at Uran, Maharashtra (west coast of India) was studied. All animals collected were associated with more than one Vibrio species. Bacterial identification based on morphological and biochemical characteristics showed that four groups of Vibrios were present in shrimp hepatopancreas namely Vibrio parahaemo...

White spot syndrome virus (WSSV) is the causative agent of a severe disease of cultivated shrimp. Using purified WSSV virions, VP53A encoded by open reading frame wssv067 was identified as a structural protein by SDS-PAGE and proteomics. Immunoelectron microscopy with a gold-labeled secondary antibody revealed that VP53A was distributed on the viral envelope. In order to further explore the lin...

A cDNA microarray composed of 2,028 different ESTs from two shrimp species, Penaeus monodon and Masupenaeus japonicus, was employed to identify yellow head virus (YHV)-responsive genes in hemocytes of P. monodon. A total of 105 differentially expressed genes were identified and grouped into five different clusters according to their expression patterns. One of these clusters, which comprised fi...

Here we used both microsatellites and mtCR (mitochondrial DNA control region) sequences as genetic markers to examine the genetic diversity and population structure of Penaeus monodon shrimp from six Indonesian regions. The microsatellite data showed that shrimp from the Indian and the Pacific Ocean were genetically distinct from each other. It has been reported previously that P. monodon mtCR ...

We report on the construction of sex-specific high-density linkage maps and identification of sex-linked markers for the black tiger shrimp (Penaeus monodon). Overall, we identified 44 male and 43 female linkage groups (2n = 88) from the analysis of 2,306 AFLP markers segregating in three full-sib families, covering 2,378 and 2,362 cM, respectively. Twenty-one putatively homologous linkage grou...

Yellow head virus (YHV) is an invertebrate nidovirus which causes a severe mortality in cultured Penaeus monodon. The mortality may be prevented by prior treatment of shrimps with YHV-protease dsRNA. Whether the YHV infected shrimp might be cured by the dsRNA remains to be investigated. P. monodon injected with 10(-6) YHV showed a high virus replication and mortality within 2 days. Injection of...

Hemocyanin sequences allineament shows the presence of highly invariant regions especially in the active site and in the tight intersubunits interaction sites. Comparing the aminoacids in contact regions between monomers is possible to interpret the stability of hexamers.

This study presents updated information on six exotic aquatic species (five crustaceans and one fish): Macrobrachium rosenbergii, Penaeus monodon, Charybdis hellerii, Balanus trigonus, Amphibalanus improvisus, Pterois volitans. The research is based bibliographic available in scientific articles published by the Research Group Fishing Evaluation of Tropical Fishery Resources Biological Photogra...

A study was carried out to evaluate polymerase chain reaction (PCR) for the detection of white spot syndrome virus (WSSV) in postlarvae and broodstock of Penaeus monodon and in potential crustacean carriers from India. Primer pairs designed for WSSV affecting Penaeus japonicus (WSSV PJ) and P. monodon (WSSV PM) were used. Both the primer pairs gave positive assay results for crustaceans showing...