In order to carry out studies on structure and function relationships of porcine pepsinogen using site-directed mutagenesis approaches, the cDNA of this zymogen was cloned, sequenced, expressed in Escherichia coli, and the protein refolded, and purified to homogeneity. Porcine pepsinogen cDNA, obtained from a lambda gt10 cDNA library of porcine stomach contains 1364 base pairs. It contains lead...

The relation between Helicobacter pylori (H pylori) infection and fasting gastrin and pepsinogen-I and -II concentrations was evaluated in 278 volunteers without symptoms and the results were compared with the values obtained in 35 patients with duodenal ulcers. H pylori infection was determined with the 13C-urea breath test in subjects without symptoms and with endoscopy, biopsy (histology and...

BACKGROUND Atrophic gastritis is a precancerous condition, which can be diagnosed by several methods. However, there is no consensus for the standard method. The aim of this study was to evaluate the correlations among endoscopic, histologic, and serologic findings for the diagnosis of atrophic gastritis. METHODS From March 2003 to August 2013, a total of 2,558 subjects were enrolled. Endosco...

background: currently, non-invasive methods for screening atrophic gastritis and gastric cancer are lacking. the purpose of this study was to evaluate the value of serological parameters including serum pepsinogen i (pgi), pepsinogen ii (pgii) and pepsinogen i: ii ratio for the screening atrophic gastritis and gastric cancer.   methods : the study population consisted of 132 dyspeptic patients ...

background h. pylori infection in the stomach was the first major cause of gastritis and peptic ulcer disease and gastric adenocarcinoma and lymphoma (malt). evaluation of the infection eradication is important. h. pylori infection was associated with gastric glands dysfunction such as increased serum gastrin and increased secretion of pepsinogen. in recent years the measurement of serum gastri...

BACKGROUND Although accumulating evidence suggests that Helicobacter pylori plays a role in gastric carcinogenesis, the magnitude of the risk remains uncertain. AIM We aimed to estimate the magnitude of the risk of gastric cancer associated with H. pylori infection by a large case-control study nested within a prospective cohort. Possible effect modification by CagA status, and serum pepsinog...

Background/Aims Gastric juice plays a crucial role in the physiology of the stomach. The aim of this study is to evaluate associations among the pH of gastric juice, atrophic gastritis (AG), intestinal metaplasia (IM), pepsinogen, and Helicobacter pylori infection. Methods Gastric biopsies and juice were collected from 46 subjects who underwent endoscopies at Seoul National University Bundang...

BACKGROUND/AIMS Synchronous/metachronous gastric epithelial neoplasias (GENs) in the remaining lesion can develop at sites other than the site of endoscopic resection. In the present study, we aimed to investigate the predictive value of serum pepsinogen for detecting multiple GENs in patients who underwent endoscopic resection. METHODS In total, 228 patients with GEN who underwent endoscopic...

BACKGROUND Helicobacter pylori (HP) infection is implicated in gastric and extra-gastric diseases. While gastritis-related chronic inflammation represents a known trigger of metabolic disturbances, whether metabolic syndrome (MetS) is affected by gastritis status remains unclear. We aimed to clarify the effect of HP-related gastritis on the risk of MetS. MATERIALS AND METHODS We retrospective...