Our study was to develop a rapid and successful method for promoting asymbiotic seed germination and growth and development of protocorm like bodies (PLBs) and subsequent plantlets of Phalaenopsis orchid. Four basal media i.e. Murashige and Skoog (MS), half strength MS (½MS), Knudson C (KC) and, Vacin and Went (VW) supplemented with coconut water and peptone were examined for their effectivenes...

Bacillus sp. can biosynthesize fibrinolytic enzymes. Culture conditions and nutrient sources are important factors affecting microbial growth production of the Fibrinolytic enzyme. Therefore, we investigated culture strain ES4, such as temperature, pH, C source (glucose, sucrose, glycerol, maltose), N (yeast extract, meat content, peptone, tryptone), metal ions (Ca 2+ , Mg Mn Zn K + Fe Cu ), ti...

Although serum-free media have been widely used in mammalian cell culture for therapeutic protein production, the effects of serum-substitutes on product quality have not been extensively examined. This study observed an adverse effect of Primatone RL, an animal tissue hydrolysate commonly used as a serum-substitute to promote cell growth, on sialylation of interferon-gamma (IFN-gamma) derived ...

Taste receptors on enteroendocrine cells sense nutrients and transmit signals that control gut hormone release. This study aimed to investigate the amino acid (AA) sensing mechanisms of the ghrelin cell in a gastric ghrelinoma cell line, tissue segments and mice. Peptone and specific classes of amino acids stimulate ghrelin secretion in the ghrelinoma cell line. Sensing of L-Phe occurs via the ...

Previous observations on the effect of oral magnesium and peptone on thrombin generation time are examined. The smallest amounts of magnesium and peptone regularly producing a significant shift demonstrable after one hour are 0.25 g. magnesium and 15 g. peptone. The oral effect can be reproduced by the intravenous injection of magnesium. The minimum amount required is 50 mg. The effect is trans...

The role of secretin in the inhibition of gastric acid secretion that occurs during acidification of the gastric lumen was studied in nine healthy men. Gastric acid secretion was stimulated by 500-ml meals of 8% peptone solution, and the pH of the stomach was maintained at 5.5, 2.5, or 2.0 by intragastric titration. The increase in plasma secretin was measured, after extraction, by a new secret...