EnzymeAssay Bovine heart CAMP-PDEactivity was measured as follows: The reaction mixture contained in a final volume of 1.0 ml, Tris-HCl buffer (pH7.0) 40him, MgSO4 2mM, CAMP0.6mMas a substrate and the enzyme 7.5mU/ml. The reaction was started by adding the substrate. Incubation was carried out 30°C for 30 minutes, and the reaction was terminated by adding 0.1ml of 55% perchloric acid, and the m...

Fabrication of monodisperse pure silica and core-shell nanospheres (Fe2O3@SiO2 and Fe2O3/QD@SiO2) was done in straight forward one-pot method combining sol-gel chemistry and supercritical fluids. For the core-shell materials, preformed nanoparticles stabilized in hexane were dispersed in a sol containing a silicon alkoxide, water and acetone as the solvent. The precursor solution was introduced...

Abstract The imprinted polymers were prepared to absorb dichlorophen (DCP) by using mesoporous silica with ordered pores and high specific surface area. Both scanning electron microscopy transmission results suggested that the nanosphere had a periodic distribution. layer of was thin uniform. adsorption experiments showed obviously improved due presence structure. maximum capacity MSNs@MIPs at ...

We demonstrate and characterize the transfer of a levitating silica nanosphere between two optical tweezers, at low pressure. Both traps are mounted on heads fibers placed translation stages in vacuum chambers. Our setup allows to physically separate particle loading environment from experimental chamber, where second tweezer can position inside high Finesse cavity. The separation prevents spoi...

Some peptide-based drugs, including oxytocin, vasopressin, ziconotide, pramlintide, nesiritide, and octreotide, contain one intramolecular disulfide bond. A novel and reusable monodispersed silica nanosphere-supported Pt(IV) complex (SiO₂@TPEA@Pt(IV)); TPEA: N-[3-(trimethoxysilyl)propyl]ethylenediamine) was synthesized via a four-step procedure and was used for the formation of intramolecular d...

A novel structure denoted a "hollow nanosphere aggregate" is synthesized by introducing nanoscale Kirkendall diffusion to the spray pyrolysis process. The hollow Fe2O3 nanosphere aggregates with spherical shape and micron size are synthesized as the first target material. A solid iron oxide-carbon composite powder that is prepared by a one-pot spray pyrolysis process is transformed into the hol...