Background: Polyinosinic:polycytidylic acid (Poly-IC) has been used as a viral stimulus to mimic in vivo and in vitro infection induced by some viruses. Objective: To determine whether non-viable Lactobacillus casei CRL431 (LcM) can modulate the immune response induced by Poly I:C in co-culture models of peripheral blood mononuclear cells (PBMC) and A549 cells. Methods: T and NK cell activati...

BACKGROUND To investigate the role of mononuclear cells and their products on rat mesangial cell proliferation, we evaluated the effect of peripheral blood mononuclear cells (PBMC) culture supernatant of patients with IgA nephropathy on rat mesangial cells 3[H]-thymidine uptake, and measured the concentration of cytokines in the supernatant to find out which cytokine in PBMC culture supernatant...

the study was conducted to evaluate the effect of thermal stress on expression profile of genes related to apoptosis in peripartum sahiwal cows. for this, twelve pregnant dry sahiwal cows were selected from livestock research centre at national dairy research institute, karnal. the cows were divided into two groups consisting of six sahiwal cows each. cows of group i calved during thermoneutral...

Natural killer (NK) cells represent promising tools for cancer immunotherapy. We report the optimization of an NK cell activation–expansion process and its validation on clinical-scale. Methods: RPMI-1640, stem growth medium (SCGM), MACS TexMACS were used as culture mediums. Activated expanded (NKAE) obtained by coculturing total peripheral blood mononuclear (PBMC) or CD45RA+ with irradiated K5...

OBJECTIVE To compare expression of the p75 chain of the interleukin-2 receptor (IL-2Rp75, CD122) on peripheral and synovial mononuclear cells in rheumatoid and non-rheumatoid inflammatory arthritis. METHODS Peripheral blood (PBMC) and synovial (SFMC) mononuclear cells were isolated from subjects with rheumatoid arthritis (n = 16) and non-rheumatoid inflammatory arthritis (n = 12). PBMC were i...

Background: Lymphadenitis is the most common complication of BCG vaccination in children. Interferon-gamma (IFN-γ) plays a key role in immune response to Mycobacterial infections. In this study, the relationship of serum levels of IFN-γ and its receptor (CD119) to development of Bacillus Calmette Guerin (BCG) axillary lymphadenopathy was investigated. <em...

People with diabetes mellitus have an increased risk for infections, however, there is still a critical gap in precise knowledge about altered immune mechanisms this disease. Since diabetic INS C94Y transgenic pigs exhibit elevated blood glucose and stable phenotype soon after birth, they provide favorable model to explore functional alterations of cells early stage vivo . Hence, we investigate...

Objective(s): The present study aimed to evaluate the receptor of advanced glycation end-products (RAGE), NF-kB, NRF2 gene expression, and RAGE cell distribution in peripheral blood mononuclear cells (PBMC) in subjects with obesity and IR compared with healthy subjects.Materials and Methods: The mRNA expression levels of RAGE, NF-kB, NRF...