The P2X7 subtype holds a special position among P2X receptors because of its ability to act both as a classical, ligand-gated ion channel, and as a permeabilization pore that can induce cell death under prolonged activation by ATP. We have shown previously that, in rat retina, P2X7 receptors are located in the inner nuclear layer and ganglion cell layer (GCL). The present study was aimed at fin...

We present a method for permeabilizing cells using a disposable substrate coated with gold nanoparticles. Upon laser irradiation, the unique photothermal properties of the gold nanoparticles facilitate selective permeabilization of the cellular membrane using patterned laser images. The permeabilization of the cell membrane is characterized by intercalation of fluorescent Calcein dye.

Enterovirus nonstructural 2B protein is involved in cell membrane permeabilization during late viral infection. Here we analyze the pore forming activity of poliovirus 2B and several of its variants. Solubilization of 2B protein was achieved by generating a fusion protein comprised of poliovirus 2B attached to a maltose-binding protein (MBP) as an N-terminal solubilization partner. MBP-2B was a...

The primary objective of this research work was to study the antibacterial effects Cupressus funebris essential oil (EO) against various drug resistant bacterial pathogens along with studying molecular docking interactions major components EO key proteins/enzymes. Gas chromatography-mass spectrometry used analyse chemical composition EO. initial screening performed by using disc diffusion and m...

After 24 h of chemical permeabilization with 20% (v/v) methanol at 25 °C, the amount of daidzein released from soybean seeds is 15 to 20% of the amount (0.0423 6 0.0045 mg/g seed dry wt) obtained by physical grinding. With this chemical permeabilization condition, 70% of the permeabilized seeds are still able to germinate. The release of daidzein is enhanced to 33% with the addition of XAD-4 to...

in this work, designs for planar, interdigitated circular electrode structures for cell membrane permeabilization procedures are analysed using finite element method analysis. The generated electric field intensity, homogeneity and the Joule heating are evaluated. The optimal electrode configuration for successful transfection procedures is investigated. Based on the simulation results, an opti...