OBJECTIVES This study was undertaken to examine the effect of oxidant on lipid peroxidation and lethal cell injury in rat liver slices. METHODS t-Butylhydroperoxide (t-BHP) was employed as a model of an oxidant. The lipid peroxidation and lethal cell injury were estimated by measuring the formation of malondialdehyde (MDA) and lactate dehydrogenase (LDH) release, respectively. RESULTS t-BHP...

Lipid peroxidation and haemoglobin degradation were the two extremes of a spectrum of oxidative damage in red cells exposed to t-butyl hydroperoxide. The exact position in this spectrum depended on the availability of glucose and the ligand state of haemoglobin. In red cells containing oxy- or carbonmono-oxy-haemoglobin, hexose monophosphate-shunt activity was mainly responsible for metabolism ...

Lipid peroxidation is known to affect the activity of several enzymes including microsomal enzymes such as glucose-6-phosphatase; but its effect on the enzymes of lipid biosynthesis has not been investigated. Glycerol-3-phosphate acyltransferase (GPAT) represents the first committed step and probably the rate limiting step in glycerolipid synthesis and thus may be a good candidate for study. Ra...

Under the influence of pathological factor, there is a change in intensity lipid peroxidation, accumulation blood concentration products free radical oxidation and reactive oxygen species, decrease buffer capacity relative to maintenance optimal parameters extreme reactions. The work aimed investigate effect feed additive “Sylimevit” on antioxidant protection body dogs after preventive dewormin...

this study was designed to explore the protective effects of ascorbic acid and water extract of spirulina platensis (blue green algae) on methotrexate-induced lipid peroxidation using goat liver as the lipid source. this in vitro evaluation was done by measuring the malondialdehyde, 4-hydroxy-2-nonenal, reduced glutathione and nitric oxide content of tissue homogenates. the study reveals the li...

malondialdehyde (mda) is widely used as a biomarker for assessing oxidative stress in biomedical fields. lipid peroxidation is a chain phenomenon resulting in the formation of various active compounds that result in cellular damage. biomonitoring of mda has been used in both in-vivo and in-vitro studies as a key biomarker for various disease patterns including hypertension, diabetes, atheroscle...

aloe vera has been used worldwide in pharmaceutical, food and cosmetic industries due to the plethora of biological activities of its constituents. this study was done to evaluate the effects of dietary aloe vera on growth and lipid peroxidation in rainbow trout (oncorhynchus mykiss). a total number of 480 o. mykiss (mean weight 9.50 ± 0.85 g) were randomized into four experimental groups inclu...

the objective of this study was to evaluate effect of different concentrations of catalase in two extenders on motility, viability and lipid peroxidation bull spermatozoa during semen freezing process. thirty ejaculates collected from ten holstein bulls were pooled and evaluated at 37 °c. pool ejaculated was split into two main experimental groups, 1 and 2. in experiment 1, specimen was diluted...