AIM This study was undertaken with the aim to compare and establish the genetic relatedness between classical swine fever virus (CSFV) genogroup 2.2 isolate and pestivirus reference strains. MATERIALS AND METHODS The available complete genome sequences of CSFV/IND/UK/LAL-290 strain and other pestivirus reference strains were retrieved from GenBank. The complete genome sequence, complete open ...

  Twelve (Experiment I) and four (Experiment II) multiparous dairy cows seronegative to pestivirus were selected and randomly assigned to either a control group which did not become infected or a treatment group in which all cows became infected following intranasal inoculation 9 days before AI. The experimental induction of infection was carried out with 2 ml of non-cytopathic pestivirus (BVD ...

  Twelve (Experiment I) and four (Experiment II) multiparous dairy cows seronegative to pestivirus were selected and randomly assigned to either a control group which did not become infected or a treatment group in which all cows became infected following intranasal inoculation 9 days before AI. The experimental induction of infection was carried out with 2 ml of non-cytopathic pestivirus (BVD ...

Inter-species transmission is often incriminated in the epidemiology of Pestivirus diseases. The purpose of this study was to investigate the prevalence of Pestivirus in some mountain wild ungulates and to determine their role in Pestivirus transmission, as mountain pastures are a place where cohabitations between wild and domestic ungulates are particularly high. Between 2003 and 2007, a longi...

Peptides deduced from the C-terminal end (residues 191 to 227) of pestivirus envelope protein E(rns) were used to develop enzyme-linked immunosorbent assays (ELISAs) to measure specifically antibodies against different types of pestiviruses. The choice of the peptide was based on the modular structure of the E(rns) protein, and the peptide was selected for its probable independent folding and g...

The positive-strand RNA genome of pestiviruses contains a single large open reading frame (ORF) extending its entire length and is capable of encoding 450 kDa of protein. Studies have been undertaken with the purpose of elucidating the specific mechanisms involved in the biogenesis of the complete complement of pestivirus proteins. Here, we report on gene expression at the 5' end of the genome ...