Recombinant DNA research requires new and versatile cloning vectors. Many of the popular plasmid vectors, such as the pUC (4), pTZ (Amersham, Arlington Heights, IL, USA) and pGEM (Promega, Madison, WI, USA) series, share common characteristics, including high copy number, sizes of around 3 kbp and the presence of a multiple cloning sequence (MCS) within the coding sequence for a part of the la...

A eukaryotic cambialistic superoxide dismutase (SOD) has been purified to homogeneity from mature seeds of the disease- and insect-resistant camphor tree (Cinnamomum camphora). Besides the known role of this SOD in protecting cells against oxidative stress, it can induce the cleavage of supercoiled double-stranded DNA into nicked and linear DNA. It can not cleave linear DNA or RNA, demonstratin...

Incubation in HeLa nuclear extract of a ^P-labeled 61 nucleotlde-tong RNA corresponding to the lariat branch site/polypyrlmldlne tract/3' splice site of the first Intron of human /S-globin pre-mRNA led to the crosslinking of a single protein of -62,000 mol. wt. (p62). p62 corresponds to a polypyrimidine tract-binding protein recently described by Garcia-Blanco et al. (Genes & Dev. 3: 1874-1886,...

Background and Aims: In humans the group A rotaviruses are associated with endemic diarrhea in children under the age of 5, leading to approximately 800,000 deaths every year. Introduction of rotavirus vaccines into childhood immunization programs can contribute to substantial reduction in mortality from rotavirus gastroenteritis in developing countries and virtually eliminating hospitalization...

A highly efficient cloning vector was constructed for cloning PCR products by inserting an 80 bp DNA fragment into pGEM-5zf (+) vector. The Xcm I digestion of this vector gave rise to a 3' overhanging deoxythymidine offering the possibility of cloning PCR products with 3' adenosine overhang created by Taq DNA polymerase. Furthermore, two EcoR I sites were added to the construct for identificati...

A new symmetric local projection method built on residual bases (RELP) makes linear equal-order finite element pairs stable for the Darcy problem. The derivation is performed inside a Petrov-Galerkin enriching space approach (PGEM) which indicates parameter-free terms to be added to the Galerkin method without compromising consistency. Velocity and pressure spaces are augmented using solutions ...

Antigen uptake by dendritic cells (DCs) is a key step for initiating antigen-specific T cell immunity. In the present study, novel synthetic polymeric nanoparticles were prepared as antigen delivery vehicles to improve the antigen uptake by DCs. Well-defined cationic and acid-responsive copolymers, monomethoxy poly(ethylene glycol)-block-poly(2-(diisopropyl amino) ethyl methacrylate)-block-poly...