Idiopathic pulmonary fibrosis (IPF) is a chronic, progressive disease characterized by fibroblast proliferation and excess deposition of extracellular matrix proteins in the lungs. As antiinflammatory therapies such as steroids or immunosuppressive agents do not improve disease progression, targets to block fibrogenesis per se are needed. Mice lacking cytosolic phospholipase A2, a key enzyme tr...

Polychlorinated biphenyls (PCBs) and phytoestrogens were found to affect contractions of bovine uterus. Prostaglandins (PG) F2α and E2 are also involved in the uterine contractility. Hence the aim of these studies was to investigate the effect of PCBs and some phytoestrogens on PG secretion from endometrial cells obtained on days 1–5 of the oestrous cycle. Cells were incubated in aerated atmosp...

OBJECTIVE Postpartum hemorrhage is most common cause of maternal mortality. Active management of third stage of labor minimizes the risk of postpartum hemorrhage. To compare the effect of methylergonovine and 15-methyl prostaglandin F2α (15-methyl PGF2α) in active management of third stage of labor. METHODS A randomized open labelled parallel study with 50 women in normal labor, 25 in each gr...

The present study was performed to determine the effect of PGF2α and GnRH injections in different time intervals on fertility in Hair goats during breeding season. Totally, 80 primiparous Hair goats were divided into four groups (n=20 in each) according to the body condition scores of the animals. The animals in groups were treated as follows; first group (Ovsynch), GnRH on day 0, PGF2α on day ...

OBJECTIVE To investigate the potential of 8-iso-prostaglandin F2α (8-iso-PGF2α) as a biomarker for disease activity and oxidative stress in the CSF of patients with multiple sclerosis (MS). METHODS The isoprostane 8-iso-PGF2α is an established biomarker for in vivo oxidative stress and lipid peroxidation. We measured CSF 8-isoPGF2α levels in 231 patients with MS (74 with relapsing-remitting M...

Bovine embryos (day 5) were cultured to day 10 with or without 100 ng/mL PGF2α in medium supplemented with control; 100 nM Dex; 1,000 U/mL recombinant human leukemia inhibitory factor (rhLIF); or Dex+rhLIF. Although the rates to development to the blastocyst were not significantly different among groups, the hatching rate after additional culture with Dex +/or rhLIF was significantly higher in ...

Suckled Bos indicus  Bos taurus cows were used to compare a Select Synch/CIDR + timed artificial insemination (AI) protocol (SSC) vs a modified Co-Synch/CIDR protocol (COS). Five groups of suckled Bos indicus  Bos taurus cows were evaluated (n=659). Cows received GnRH and a CIDR on d 0. On d 7, SSC cows had their CIDR removed and received prostaglandin F2α (PGF2α), whereas COS cows had their ...

OBJECT Platelet isoprostane 8-ISO-prostaglandin F2α (8-iso-PGF2α), a proaggregating molecule, is believed to derive from nonenzymatic oxidation of arachidonic acid. We hypothesized that NADPH is implicated in isoprostane formation and platelet activation. METHODS AND RESULTS We studied 8-iso-PGF2α in platelets from 8 male patients with hereditary deficiency of gp91(phox), the catalytic subuni...

The aim of the study was to estimate association of the extent of angiographically proven coronary artery disease (CAD) with plasma 8-isoprostane F2 (8-iso-PGF2α) levels as a reliable marker of lipid peroxidation and serum activity of paraoxonase-1, which demonstrates the ability to protect against lipid oxidation. The study included 105 patients with angiographically documented CAD (CAD+) and ...

The notion that oxidative stress plays a role in virtually every human disease and environmental exposure has become ingrained in everyday knowledge. However, mounting evidence regarding the lack of specificity of biomarkers traditionally used as indicators of oxidative stress in human disease and exposures now necessitates re-evaluation. To prioritize these re-evaluations, published literature...