The well-characterized plasmid-encoded naphthalene degradation pathway in Pseudomonas putida PpG7(NAH7) was used to investigate the role of the NAH plasmid-encoded pathway in mineralizing phenanthrene and anthracene. Three Pseudomonas strains, designated 5R, DFC49, and DFC50, were recovered from a polynuclear aromatic hydrocarbon-degrading inoculum developed from a manufactured gas plant soil s...

Rieske oxygenase (RO) systems are two- and three-component enzyme systems that catalyze the formation of cis-dihydrodiols from aromatic substrates. Degradation of pollutants in contaminated soil and generation of chiral synthons have been the major foci of RO research. Substrate specificity and product regio- and stereoselectivity have been shown to vary between individual ROs. While directed e...

This study focuses on the isolation and biodegradation of PAHs by microorganisms associated with the sediments of automobile workshops of Pondicherry. One of the bacterial isolate selectively enriched in these compounds was identified as Rhodococcus ruber based on morphology, 16S rDNA sequencing and phylogenetic characteristics. Biodegradability tests with selected PAHs supplied as sole carbon ...

A series of meso arylethynyl BODIPYs (2a-2h) were designed and synthesized by the Pd-catalyzed Sonogashira cross-coupling reaction. The effects of the donor on the photophysical properties of the BODIPYs were explored. The DFT optimized structures and crystal structures show the planar orientation of the donor group with respect to the acceptor BODIPY, which favors a high degree of conjugation ...

A laboratory-scale drum bioreactor system was used to study engineering aspects of soil bioremediation. Polycyclic aromatic hydrocarbons (PAHs) were chosen as contaminants in soil. In the operation of the reactor, different mixing strategies were applied according to the size of soil without separate washing of sand. The effect of the water content of the soil mixture on solid mixing time and p...

The hydrogen-abstraction/acetylene-addition (HACA) mechanism has been central for the last decades in attempting to rationalize the formation of polycyclic aromatic hydrocarbons (PAHs) as detected in carbonaceous meteorites such as in Murchison. Nevertheless, the basic reaction mechanisms leading to the formation of even the simplest tricyclic PAHs like anthracene and phenanthrene are still elu...

A bacterial strain P2 capable of using phenanthrene as a sole source of carbon and energy was isolated from a lubricant-contaminated soil sample collected from a garage in Prajinburi province, Thailand and identified by 16S rRNA gene sequence analysis to be in the genus of Sphingomonas. The Sphingomonas sp. strain P2 rapidly degraded phenanthrene from 100 mgl to undetectable level by HPLC analy...

The expansion of a microbial bank for the degradation of polycyclic aromatic hydrocarbons (PAHs) is crucial for removal of these persistent pollutants. In this study, five gram-negative, aerobic, non-fermentative bacterial strains (III-R3, IV-P11, IV-P13, IV-R13, and V-P18) were isolated from the activated sludge of a petrochemical wastewater treatment plant using enrichment pro­ tocol based on...

the expansion of a microbial bank for the degradation of polycyclic aromatic hydrocarbons (pahs) is crucial for removal of these persistent pollutants. in this study, five gram-negative, aerobic, non-fermentative bacterial strains (iii-r3, iv-p11, iv-p13, iv-r13, and v-p18) were isolated from the activated sludge of a petrochemical wastewater treatment plant using enrichment pro­ tocol based on...