Abstract Human γδ T cells represent a small fraction of in peripheral blood (1–10%). They constitute unique subset lymphocytes that recognize non-peptide antigens through MHC-independent presentation. Major cell subsets, Vδ1 and Vδ2 possess distinct tissue localization, antigen recognition, effector responses. We hypothesized differences at the gene, phenotypic, functional level distinguish the...

Candida auris is an emerging multidrug-resistant fungal pathogen causing nosocomial and invasive infections associated with high mortality. C. auris is commonly misidentified as several different yeast species by commercially available phenotypic identification platforms. Thus, there is an urgent need for a reliable diagnostic method. In this paper, we present fast, robust, easy-to-perform and ...

OBJECTIVES To compare genotypic and phenotypic HIV-1 drug resistance assays. METHODS Protease inhibitor (PI) susceptibility was phenotypically analysed in HIV-1 isolates and recombinant viruses expressing proteases from viral isolates. Two genotypic interpretation methods were carried out in parallel. RESULTS Entirely concordant resistance levels were shown in 5/10 (50%) highly active anti-...

A fraction of a genetically homogeneous microbial population may survive exposure to stress such as antibiotic treatment. Unlike resistant mutants, cells regrown from such persistent bacteria remain sensitive to the antibiotic. We investigated the persistence of single cells of Escherichia coli with the use of microfluidic devices. Persistence was linked to preexisting heterogeneity in bacteria...

The performance of hybrids relative to their parents is an important factor in speciation research. We measured the growth of 46 Saccharomyces yeast F1 interspecific and intraspecific hybrids, relative to the growth of each of their parents, in pairwise competition assays. We found that the growth of a hybrid relative to the average of its parents, a measure of mid-parent heterosis, correlated ...

in order to characterizeblackleg and soft rot agent of potatoin ardabil province, samples showing mentioned symptoms were gathered from fields and storages during 2012 and 53 pectolytic isolates were obtained in primary purification. after confirming their pathogenicity, phenotypic features were investigated and compared to those of the reference strains. rotting severityof isolates and infecti...

Antiviral drug resistance can be one of the causes for HIV-1 therapy failure. Several studies have shown some beneficial effect of antiviral resistance testing on response to the following therapy regimen. The technical performances of genotypic and phenotypic assays have been considerably improved with time. However, both are still limited in their power to fully map antiviral resistance as ca...

The usefulness of ultra-deep pyrosequencing (UDPS) for the diagnosis of HIV-1 drug resistance (DR) remains to be determined. Previously, we reported an explosive outbreak of HIV-1 circulating recombinant form (CRF) 07_BC among injection drug users (IDUs) in Taiwan in 2004. The goal of this study was to characterize the DR of CRF07_BC strains using different assays including UDPS. Seven CRF07_BC...

Human immunodeficiency virus type 1 (HIV-1) isolates from 50 plasma specimens were analyzed for phenotypic susceptibility to licensed reverse transcriptase inhibitors and protease inhibitors by the Antivirogram and PhenoSense HIV assays. Twenty of these specimens were from recently seroconverted drug-naïve persons, and 30 were from patients who were the sources of occupational exposures to HIV-...

We have compared molecular, immunochemical, and cytotoxic assays for the detection of diphtheria toxin from 55 isolates of Corynebacterium diphtheriae and Corynebacterium ulcerans originally isolated in five different countries. The suitabilities and accuracies of these assays for the laboratory diagnosis of diphtheria were compared and evaluated against the "gold standard" in vivo methods. The...