Euglena gracilis was grown in the dark for 12 days. Subsequent incubation with sodium octanoate-l-14C in the dark for 6 hours resulted in a rather specific incorporation of radioactivity into the neutral lipids (primarily wax esters). Upon illumination of these cells in an identical medium without radiocarbon, the radioactivities in the neutral lipids decreased strongly, due to a decrease of th...

Laser Raman spectroscopy has been employed to investigate the effects of cyclosporine-A on the order and dynamics of DPPC (Dipalmytoyl Phosphatidyl Choline) midel membrane system. It is shown that the addition of small amount of cyclosporine-A to a DPPC dispersion disturb the system and changes the order/disorder parameter of the model membrane.

The isolation of phosphatidyl choline which contains all of its nitrogen as choline, phosphorus as glycerophosphate, and has a relatively high iodine number, has posed a problem for some time. The most widely used method has been that of Levene and Rolf (l), as modified by Pangborn (24), wherein this lipide is isolated through a cadmium chloride complex. Sinclair (5) has described a procedure f...

Pulmonary Surfactant, Type II Cells, Biosynthetic Pathways, Isotopic Dilution, Serum Hormones Using radiolabels, we studied the effect of certain experimental conditions on the incorporation of choline and palmitate into phosphatidyl choline (lecithin) of cloned type II rat lung cells. When the label was changed from methyl-3H to 1,2-14C the incorporation of choline was reduced to 1/3; in contr...

After the intramuscular administration of 100,000 LU. of retinol (Prepalin) daily for 13 days to a group of young rats, their daily food consumption, body weights, total spleen lipids, total spleen cholesterol and phospholipid phosphorus did not differ significantly from a control group. A significant enlargement of spleen was observed in the treated rats. A significant increase in the con cent...

Microelectrophoresis was found to be a rapid method for studying the interaction between aminoglycoside antibiotics and liposomes prepared from acid phospholipids. The ability to cause charge reversal of liposomes prepared from phosphatidyl inositol was ranked in the order neomycin greater than gentamicin greater than tobramycin greater than amikacin greater than kanamycin greater than ribostam...

The microviscosity and fluidity of erythrocyte ghost membranes from lead workers and control subjects was measured by fluorescence polarisation using the fluorophore, 1,6-diphenyl-1,3,5-hexatriene (DPH). Increased lead was associated with a significant decrease in the average microviscosity of resealed and unsealed erythrocyte membranes. Since DPH fluorescence reflects the organisation of lipid...

Phospholipid metabolism in the lung was studied because the lung was the first organ at which the ingested lipids arrived. It was revealed in this study that the lung contained phosphatidyl cho-line, ethanolamine, phosphoinositol, sphingomyelin and lysolecithin, that the amount of phosphatidyl serine were very small in the lung, and that a phosphatidic acid was discovered in the lung after the ...