Membranous material was prepared from Ehrlich ascites carcinoma cells by tonal centrifugation. The distribution of fatty acids among the individual neutral lipid and phospholipid classes isolated from the membrane preparation was determined. Survey electron microscopy indicated that the preparations consisted of vesicles of rough surfaced endoplasmic reticulum, lysosomal-like structures, virus-...

When (32)P(1) was supplied as a 15-minute pulse to normal Spirodela oligorrhiza plants, the first phospholipid to become fully labeled was phosphatidic acid. Phosphatidyl glycerol reached maximum labeling before the other major phospholipids. In phosphorus-deficient plants, however, phosphatidyl glycerol became labeled much more slowly than either phosphatidyl choline or phosphatidyl ethanolami...

Glyoxysome, endoplasmic reticulum, mitochondria, and proplastid fractions were isolated from endosperm of castor beans (Ricinus communis) germinated for 5 days at 30 C. Samples from sucrose density gradients were diluted with 0.15 m KCI and the membranes pelleted. Lipid extracts of these membranes were analyzed for phosphoglyceride, acyl lipid, and sterol content. The endoplasmic reticulum cont...

As cultures of Escherichia coli progressed from the exponential growth phase to the stationary growth phase, the phospholipid composition of the cell was altered. Unsaturated fatty acids were converted to cyclopropane fatty acids, and phosphatidyl glycerol appears to have been converted to cardiolipin. With dual isotope label experiments, the kinetics of synthesis of cyclopropane fatty acid for...

Significant differences in lipid composition have been found between normal human lymphocytes and polymorphonuclear leukocytes (isolated from blood by means of glass-bead columns), abnormal leukocytes from patients with acute and chronic leukemia, and leukocytes from peritoneal exudates. Lipid extracts of isolated leukocytes were analyzed for total lipid, phosphorus, cholesterol, and plasmaloge...

Chloroform-methanol 2:1 removes a significantly greater quantity of lipid from bleached bovine retinal rods than from a dark-adapted counterpart. The extracts contain phosphatidyl serine, phosphatidyl choline, phosphatidyl ethanolamine, sphingomyelin, and an unknown substance which, it is proposed, may be a combination of phospholipid and retinaldehyde. The difference between extracts of light-...

The acyl group of acyl dihydroxyacetone phosphate was shown to be enzymatically transferred in guinea pig liver mitochondria to various acceptors such as lysolecithin, lysophosphatidyl ethanolamine and sn-glycerol3-phosphate to form lecithin, phosphatidyl ethanolamine and phosphatidate, respectively. Coenzyme A and Mg*, but not ATP, were required for this reaction. A rapid exchange of acyl grou...