Previous studies have reported an increased turnover of phospholipid in isolated islets of Langerhans in response to raised glucose concentrations. The present investigation was thus undertaken to determine the nature of any phospholipases that may be implicated in this phenomenon by employing various radiolabelled exogenous phospholipids. Hydrolysis of 1-acyl-2-[14C]arachidonoylglycerophosphoi...

A two-stage enzymatic hydrolysis method, in which phospholipase A1 (PLA1) was added after A2 (PLA2) for a certain time, successfully carried out to prepare glycerylphosphorylcholine (GPC) from soybean concentrated phospholipid. Effects of reaction variables on were optimized using response surface methodology, and the optimal conditions as follows: PLA2 load 1.25%, PLA1 0.70%, substrate concent...

Phosphatidylinositol phosphodiesterase activated by diacylglycerol is substantially inhibited by all phospholipids containing a phosphocholine head group, including phosphatidylcholine, hydrogenated phosphatidylcholine, choline plasmalogen, lysophosphatidylcholine, lysocholine plasmalogen, sphingomyelin and sphingosylphosphocholine. The sphingosine-containing phospholipids are the most inhibito...

In this study, we maximized the reactivity of phospholipids hydrolysis with immobilized industrial-class phospholipase A1 (PLA1) at desired water content in water-in-oil (W/O) microemulsion phase. The optimal hydrophobic-hydrophilic condition reaction media a hydrophobic enzyme is critical to realize maximum yields activity A1. It was attributed enzymes disliking surroundings as special molecul...