Four toxin complexes, Tca, Tcb, Tcc and Tcd from the culture broth of Photorhabdus luminescens have been puri®ed and the four toxin complex encoding loci, tca, tcb, tcc and tcd, cloned. Genetic knockout of either tca or tcd reduced oral toxicity to Manduca sexta, and knockout of both loci eliminated activity. Puri®ed Tca speci®cally affected the insect midgut, despite its putative normal delive...

Photorhabdus luminescens secretes both high molecular weight insecticidal toxin complexes and also a range of extracellular proteases into culture broth. Previous studies by others have suggested that insecticidal activity of the broth is associated with these proteases. However, by gene cloning and targeted knock-out, we have previously shown that oral insecticidal activity is associated with ...

Photorhabdus luminescens subsp. laumondii HP88 is an entomopathogenic bacterium that forms a symbiotic association with Heterorhabditis nematodes. We report here a 5.27-Mbp draft genome sequence for P. luminescens subsp. laumondii HP88, with a G+C content of 42.4% and containing 4,243 candidate protein-coding genes.

From the characterisation, partial 16 S gene sequences obtained for two bacteria were subjected to blast-bootstrap analysis obtain phylogenetic tree. In assessing similarity of Xenorhabdus bovienii with five other spp., it was found be 96% similar X. nematophila and japonica Accession Numbers D78006 NR027194 respectively. The closer beddingii 95% kazodoii 97% poinarii. tree, species belong gene...

Quorum sensing is a typical communication system among Gram-negative bacteria used to control group-coordinated behavior via small diffusible molecules dependent on cell number. The key components of a quorum sensing system are a LuxI-type synthase, producing acyl-homoserine lactones (AHLs) as signaling molecules, and a LuxR-type receptor that detects AHLs to control expression of specific targ...

More than a quarter of the world's population is infected with nematode parasites, and more than a hundred species of nematodes are parasites of humans [1-3]. Despite extensive morbidity and mortality caused by nematode parasites, the biological mechanisms of host-parasite interactions are poorly understood, largely because of the lack of genetically tractable model systems. We have demonstrate...

The luxA, B, C, D, and E genes from Photorhabdus luminescens were cloned and functionally expressed in Saccharomyces cerevisiae to construct a bacterial lux-based yeast bioreporter capable of autonomous bioluminescence emission. The bioreporter was engineered using a series of pBEVY yeast expression vectors that allowed for bi-directional constitutive or inducible expression of the individual l...

http://dx.doi.org/10.1016/j.anbehav.2017.03.016 0003-3472/© 2017 The Authors. Published by Elsevie license (http://creativecommons.org/licenses/by/4.0/) The act of predation often comprises multiple sequential steps whereby prey can employ defences at all or some of these stages to deter predation. However, investment in defences is costly unless they are outweighed by conferring some benefit t...