In the present study, Plackett–Burman design (PBD) followed by response surface methodology (RSM) was used to optimize the culture medium composition for laccase production from Pleurotus ostreatus IMI 395545. Influence of carbon, nitrogen (sources) and inducer were evaluated by Plackett–Burman design. The factors that induce positive effect on enzyme production were further optimized using cen...

Marine actinomycetes were isolated from sediment samples collected from Pitchavaram mangrove ecosystem situated along the southeast coast of India. Maximum actinomycete population was noted in rhizosphere region. About 38% of the isolates produced L-asparaginase. One potential strain KUA106 produced higher level of enzyme using tryptone glucose yeast extract medium. Based on the studied phenoty...

234 Application of Statistical Design to Optimize Culture Medium for Xylanase Production by Bacillus pumilus AB-1 Snehal Ingale, Sonam Kalyani 1 and Urvish Chhaya Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology & Allied Sciences, New Vallabh Vidyanagar-388120, Gujarat, India Natubhai V. Patel College of Pure and Applied Sciences, Vallabh Vidyanagar-388120, Gujar...

In many industrial areas such as in food, pharmaceutical, cosmetic, printing, and textile, the use of synthetic dyes has been integral with products such as azo dye, anthrax, and dyestuffs. As such, these industries produce a lot of waste by-products that could contaminate the environment. Bioremediation, therefore, has become an important emerging technology due to its cost-sustainable, effect...

Biofuels production has come forth as a future technology to combat the problem of depleting fossil fuels. Bio-based ethanol production from enzymatic lignocellulosic biomass degradation serves an efficient method and catching the eye of scientific community. High cost of the enzyme is the major obstacle in preventing the commercialization of this process. Thus main objective of the present stu...

Among the antitumor drugs, bacterial enzyme L-asparaginase has been employed as the most effective chemotherapeutic agent in pediatric oncotherapy especially for acute lymphoblastic leukemia. Glutaminase free L-asparaginase producing actinomycetes were isolated from soil samples collected from Egypt. Among them, a potential culture, strain NEAE-119, was selected and identified on the basis of m...

Methotrexate (MTX) being the widely used biologic agent is used to treat severe and/or disabling psoriasis but precipitates various side effects on oral therapy. Encapsulating methotrexate in flexible liposomes for delivery through skin could lead to better therapeutic action and reduce side effects. In order to screen out the various factors during liposome formulation development, Plackett Bu...

A number of nutritional factors influencing glucose oxidase (EC 1.1.3.4) production by Aspergillus niger NCIM 545 were studied. The synthesis of glucose oxidase by A. niger was investigated in two steps using submerged fermentation at 30±2 °C and 180 rpm for 96 h. Primarily, nutritional components were selected by onefactor-at-a-time method, and the significance of each component with respect t...

Orthogonal Latin hypercubes (OLHs) are available for only a limited collection of run sizes. This paper presents a simple algorithm for constructing orthogonal designs that are nearly Latin hypercubes. The algorithm is based on the approach developed by Steinberg and Lin for OLH designs and can generate designs for all run sizes for which a Plackett-Burman design exists. The designs have good u...