Cotyledonary node explants excised from 21 day old seedlings of T. arjuna produced multiple shoots when cultured on full strength MS or modified MS (1/2 strength major salts and Fe-EDTA) medium supplemented with different concentrations (0.1-1.0 mg/l) of BAP. Maximum 8.9 shoots/explant could be recorded after 30 days of inoculation on modified MS medium supplemented with BAP (0.5 mg/l). A proli...

Soil or growing media salinity is an increasing problem in agriculture throughout the world. Therefore, there is a real interest in a better understanding of the mechanisms set by the plants to respond to this stress. The present study concerns tomato, which is one of the most important cultivated vegetables. Tomato seeds (Lycopersicon esculentum var. 'Moneymaker') were disinfected and a salt s...

Synthetic seed technology is an alternative to traditional micropropagation for production and delivery of cloned plantlets. Synthetic seeds were produced by encapsulating nodal segments of C. angustifolia in calcium alginate gel. 3% (w/v) sodium alginate and 100 mM CaCl2 · 2H2O were found most suitable for encapsulation of nodal segments. Synthetic seeds cultured on half strength Murashige and...

This paper documents the key anatomical features during the development of P. armeniacum zygotic embryos and their ability to germinate asymbiotically in vitro. This study also examines the effect of media and seed pretreatments on seed germination and subsequent seedling growth. Seeds collected from pods 45 days after pollination (DAP) did not germinate while 95 DAP seeds displayed the highest...

An efficient micropropagation protocol of Cyclopia genistoides (L.) Vent., an indigenous South African shrub of economic importance, was established. In vitro shoot cultures were obtained from shoot tip fragments of sterile seedlings cultured on solid Schenk and Hildebrandt (SH) medium supplemented with 9.84 microM 6-(gamma,gamma-dimethylallylamino)purine (2iP) and 1.0 microM thidiazuron (TDZ)....

As the consequence of anthropic pressure along with climatic factors, vegetation hills Bassar is degrading day after and a regression many woody species observed. Vitellaria paradoxa highly valued multi-use species, but it threatened extinction in area. The "bell" "L" shapes diameter class distribution histograms reflect aging and/or disturbed populations. Stand disturbance illustrated by low p...

The aim of the research was to determine field, greenhouse and seed bed performance of plantlets derived from in vitro propagated plantlets of three potato cultivars. Generally, higher values were obtained from the mid-early maturing cultivar Marabel, compare to other cultivars (early cultivar Velox and and mid-early cultivar Concorde) for tuber yield, tuber number and average tuber weight. For...

In vitro asymbiotic seed germination of Dendrobium chrysotoxum varied with fruit harvesting time and culture medium used for germinating seeds. Seeds at different developmental stages were harvested and cultured on five asymbiotic orchid seed germination media i.e. defined and undefined media, namely 1⁄2 MS, KC, M, MS and PDA. Seeds harvested 220 days after pollination showed maximum germinatio...

In this paper, we studied polyetheneglycol (PEG) pretreatment effect on the mature embryo culture in vitro by using barley (Hordeum vulgare L.) seeds. Meanwhile, we analyzed and assayed its mineral element and endogenous hormone level. The experimental results were as follows: (1) PEG-6000 imbibition could obviously slow down the water timecourse absorbed by barley seeds; (2) 10% PEG-6000 treat...