Minicircles are non-viral delivery vectors with promising features for biopharmaceutical applications. These plasmid-derived circular DNA molecules that obtained in vivo Escherichia coli by the intramolecular recombination of a parental plasmid, which generates minicircle containing eukaryotic therapeutic cassette interest and miniplasmid prokaryotic backbone. The production process results thu...

Gene regulation circuits control all aspects of the life of plasmids. This review gives an overview of the current orchestration of the circuits that control plasmid replication, plasmid transfer, plasmid segregation and plasmid maintenance.

background: p19 cells are mouse embryonic carcinoma cells which contain pluripotent ability, like stem cells, to differentiate into different cell lines. there are several properties for this cell line that make it a valuable cell model for study of developmental stages. methods: at the first step, pts2-egfp coding sequence which was cloned in pucd2.hygro vector was used for transfection in to ...

Background: The importance of plasmid-encoded Extended Spectrum Beta Lactamase (ESBL) organisms in the dissemination multi-drug resistance and complicated infections are contributory factors to treatment failure. Despite these, there is a paucity information regarding plasmid-borne associated infection from primary sources including cockroaches while studies correlating total protein ESBL also ...

ABSTRACT Plasmids are common in natural bacterial communities, facilitating evolution via horizontal gene transfer. Bacterial species vary their proficiency to host plasmids: whereas plasmids stably maintained some regardless of selection for plasmid-encoded genes, other species, even beneficial rapidly lost. It is, however, unclear how this variation affects plasmid persistence communities. He...

Some years ago, we found that Axial organ (AO) cells exerted an induced and spontaneous cytotoxicity against SP2 MBL2 mouse tumoral cells. Recently, discovered a sea star Igkappa gene with immune properties. This was first, inserted in CMV (cytomegalovirus) finally plasmid called « young » plasmid. In the present experiment IGKappa cloned HeK human to produce specific protein. The induced« prot...

Introduction: Multiple sclerosis (MS) is a disease of the immune system: it attacks the myelin around the axons and leaves them exposed. Destruction of myelin weakens the electrical conduction of ions and thus leads to a lack of communication in the nervous system.  Methods: In the present study, we constructed recombinant plasmid and then transformed to E. coli cell. The colonies containing...

Nonpolar virB mutants of Agrobacterium tumefaciens were tested for RSF1010 mobilization and extracellular complementation. virB2 to virB11 were essential for transfer in both assays. virB1 was essential only for high frequency transfer of RSF1010 and VirE2. Coordinated transfer of a preassembled T complex is supported by these data and competition studies.

Colistin is one of the last-resort therapeutic antibiotics used to control carbapenem-resistant Klebsiella pneumoniae. However, emergence colistin-resistant strains with high mortality and morbidity has become a new challenge. Therefore, it crucial characterize genotypic features carbapenem- K. pneumoniae (CCKP) isolates implement public health intervention strategies. In this study, we analyze...