The yeast, Saccharomyces cerevisiae, was used as a model to investigate theories of ploidy evolution. Mutagenesis experiments using the alkylating agent EMS (ethane methyl sulphonate) were conducted to assess the relative importance that masking of deleterious mutations has on response to and recovery from DNA damage. In particular, we tested whether cells with higher ploidy levels have relativ...

Different ploidy levels, diploid (2n=2x=24) to hexaploid (2n=6x=72) have been reported for Acorus calamus, a perennial medicinal plant. According to available information, there is a significant relationship between the ploidy level and essential oil compositions and medicinal properties of this important genetic resource. However, there is no information about the chromosome number of A. calam...

To investigate the patterns of DNA ploidy and proliferating activity in breast cancer and relate them to other prognostic factors, paraffin blocks of 53 cases of breast carcinoma were studied. Cancer cells obtained by mechanical tissue disaggregation were examined for DNA content, ploidy and S-phase fraction. DNA assay was done using a CAS interactive image analyzing system. All of the cas...

BACKGROUND The optimal approach to diagnosis and treatment of localized prostate cancer remains controversial. Deoxyribonucleic acid (DNA) ploidy has been suggested as an important predictor for outcome in prostate cancer. The purpose of this study was to correlate DNA ploidy with disease-specific survival in patients with clinically localized prostate cancer treated with no intent to cure. M...

Few studies have assessed how ploidy type within a species affects genetic variation among populations in relation to source climates. Basin wildrye (Leymus cinereus (Scribn. & Merr.) A. Love) is a large bunchgrass common in the intermountain Western U.S. found in both octoploid and tetraploid types. In common gardens at two sites over two years differences in both ploidy type and genetic varia...

Microdensitometric measurement of the DNA content of individual megakaryocytes was performed using megakaryocyte colonies obtained following culture, in soft agar, of hematopoietic cells from C57BL/6J mice. Two types of colonies were detected. After 7 days of culture, the big cell type contained 16 /+- 2.3 acetylcholinesterase (AChE) positive cells/colony, with a mean ploidy level of 16.8 /+- 0...

OBJECTIVES The common subtypes of renal tumors are conventional or clear cell carcinoma, papillary carcinoma, chromophobe carcinoma and oncocytoma. Each subtype has its distinct histogenesis and clinical evolution. DNA ploidy is viewed as a marker of gross genomic aberrations. The aim of this study is to evaluate the DNA ploidy in the common subtypes of renal tumors to increase our understandin...

ABSTRACT. The genus Magnolia includes over 250 species that range in ploidy level from diploid to hexaploid. Although there is basic information on ploidy levels of various species, sampling has been limited and little information on specific cultivars and hybrids is available. The objective of this research was to determine relative genome sizes and relationships to ploidy levels among a diver...

OBJECTIVE We investigated the correlation between ploidy or S-phase fraction (SPF) and the clinical pathological characteristics of patients with peritoneal carcinomatosis from ovarian cancer. We also assessed their relation with the in vivo and in vitro response to several chemotherapeutic agents. PATIENTS AND METHODS Fifty-three patients with peritoneal carcinomatosis from ovarian cancer we...

ploidy with a DNA index of 1.9 was detected in only 1 patient. This patient, who had a stage IA serous BOT, is alive without any recurrence after 96 months of observation following TAH/BSO. All the remaining 69 tumours showed diploid DNA content. The coefficient of variation for the diploid peaks ranged from 7.48 to 12.8 (mean 8.67) in diploid histograms despite using a low flow rate. A recent ...