نتایج جستجو برای: polymerase chain reaction

تعداد نتایج: 681302  

Journal: :jundishapur journal of microbiology 0
mishar kelishadi infectious diseases research center, golestan university of medical sciences, gorgan, ir iran mandana kelishadi department of ophthalmology, 5th azar hospital, gorgan, ir iran abdolvahab moradi infectious diseases research center, golestan university of medical sciences, gorgan, ir iran naeme javid infectious diseases research center, golestan university of medical sciences, gorgan, ir iran masoud bazouri infectious diseases research center, golestan university of medical sciences, gorgan, ir iran alijan tabarraei infectious diseases research center, golestan university of medical sciences, gorgan, ir iran; infectious diseases research center, golestan university of medical sciences, gorgan, ir iran. tel: +98-1714422652, fax: +98-1714440225

results of 50 patients, 20 were men and 30 women with mean age of 61.1 ± 16.9 years ranged between 22 and 85 years. all samples of pterygia had positive results for adenoviruses dna with polymerase chain reaction, but none of the negative control groups displayed adenoviruses. the pterygium group and the control groups were β-globin positive. direct sequencing of pcr products confirmed adenovir...

Journal: :jundishapur journal of microbiology 0
mohammad soleimani department of microbiology, qom branch, islamic azad university, qom, ir iran; department of microbiology, qom branch, islamic azad university, qom, ir iran, p.o. box: 37185/364. tel:+98-2517780001, fax: +98-2517780001 mohammad reza zolfaghari department of microbiology, qom branch, islamic azad university, qom, ir iran abbas morovvati department of microbiology, qom branch, islamic azad university, qom, ir iran

background aggregatibacter actinomycetemcomitans and tannerella forsythensis are two major pathogens in destructive periodontal disease in humans. the detection of these bacteria is needed for diagnosis and management of the mentioned diseases. objectives we aimed to develop and compare improved multiplex conventional and sybr green real time pcr assays for a specific diagnosis of the organisms...

Journal: :jundishapur journal of microbiology 0
shahrzad atapoor faculty of agriculture and natural sciences, shahrekord branch, islamic azad university, shahrekord, ir iran farhad safarpoor dehkordi young researchers and elites club, shahrekord branch, islamic azad university, shahrekord, ir iran ebrahim rahimi department of food hygiene and public health, college of veterinary medicine, shahrekord branch, islamic azad university, shahrekord, ir iran; department of food hygiene and public health, college of veterinary medicine, shahrekord branch, islamic azad university, shahrekord, ir iran. tel: +98-9133278377, fax: +98-3813361060

conclusions: vegetables are a new source of h. pylori and accurate washing of vegetables improves such contaminations. results: the results showed that 44 of 460 samples (9.56%) were positive for h. pylori using the culture method. the polymerase chain reaction technique showed that 50 of 460 samples (10.86%) were positive for h. pylori. un-washed leek, traditional salad, un-washed basil and un...

Journal: :iranian journal of pediatric hematology and oncology 0
mb khalili associate professor of microbiology, dept. of microbiology, school of medicine, shahid sadoughi university of medical sc m sadeh phd student, msc of microbiology, dept. of laboratory sciences, school of paramedicine, shahid sadoughi university of meسازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید صدوقی یزد (shahid sadooghi university of medical sciences) s hekmati moghaddam assistant professor of pathology, dept. of laboratory sciences, school of paramedicine, shahid sadoughi university of meسازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید صدوقی یزد (shahid sadooghi university of medical sciences)

abstract background brucellosis is endemic in iran, and is seen in all age groups, including children. its diagnosis in childhood needs a high index of suspicion. the diagnostic methods currently in use need evaluation for analytical performance. objective the present study tries to evaluate the hematological indices, serologic markers of inflammation and symptoms in patients suspected of bruce...

Journal: :journal of research in medical sciences 0
gholamreza mohajeri mohammad reza mohajeri noushin afshar-moghaddam alireza aslanpour

background: colorectal tumor is one of the main causes of death in our country. the aim of the present study was to determine the clinicopathological aspects of tumor and the presence of hepatic micrometastasis in patients with colorectal cancer (crc). materials and methods: forty two patients with crc were evaluated in the study surgical treatment was performed and liver biopsy was taken for t...

Journal: :journal of medical bacteriology 0
leila azimi antimicrobial resistance research center, tehran university of medical sciences, tehran, ir iran and department of microbiology, faculty of medicine, tehran university of medical sciences, tehran, ir iran. abdolaziz rastegar-lari antimicrobial resistance research center, tehran university of medical sciences, tehran, ir iran and department of microbiology, faculty of medicine, tehran university of medical sciences, tehran, ir iran. malihe talebi department of microbiology, faculty of medicine, tehran university of medical sciences, tehran, ir iran. amirmorteza ebrahimzadeh-namvar antimicrobial resistance research center, tehran university of medical sciences, tehran, ir iran. somayeh soleymanzadeh-moghadam antimicrobial resistance research center, tehran university of medical sciences, tehran, ir iran.

b ackground : one of the main mechanisms of resistance to carbapenems is potential of klebsiella pneumoniae to produce k . pneumoniae carbapenemase (kpc). kpc is an important type of carbapenemase, which can hydrolyze carbapenems and other beta-lactam antibiotics. modified hodge test (mht) and  use  of  boronic  acid  as  a  kpc  inhibitor  are  two  types  of  phenotypic methods, which are use...

Journal: :iranian red crescent medical journal 0
saeid amel jamehdar department of microbiology and virology, imam reza hospital, school of medicine, mashhad university of medical sciences, mashhad, ir iran gholamali mammouri department of pediatrics, ghaem hospital, school of medicine, mashhad university of medical sciences, mashhad, ir iran mohammad reza sharifi hoseini department of pediatrics, ghaem hospital, school of medicine, mashhad university of medical sciences, mashhad, ir iran hosein nomani department of microbiology and virology, imam reza hospital, school of medicine, mashhad university of medical sciences, mashhad, ir iran monavvar afzalaghaee department of biostatistics, mashhad university of medical sciences, mashhad, ir iran hassan boskabadi department of pediatrics, ghaem hospital, school of medicine, mashhad university of medical sciences, mashhad, ir iran

conclusions: in neonates and infants with primary diagnosis of sepsis, hsv infection should be considered especially if the clinical condition does not improve after 48 hours of antibiotic therapy, and sepsis still exists with elevated liver enzymes. results: among 150 neonates and infants younger than 3 months old with sepsis, the pcr results for detecting the hsv dna, were positive in 6 sampl...

Journal: :iranian red crescent medical journal 0
emad yahaghi department of microbiology, damghan branch, islamic azad university, damghan, iran abbas ali imani fooladi applied microbiology research center, baqiyatallah university of medical sciences, tehran, ir iran; applied microbiology research center, baqiyatallah university of medical sciences, tehran, ir iran. tel: +98-9122269267 mohsen amin department of drug and food control, tehran university of medical sciences, tehran, ir iran reza mirnejad molecular biology research center, baqiyatallah university of medical sciences, tehran, ir iran reza nezamzade department of microbiology, damghan branch, islamic azad university, damghan, iran jafar amani applied microbiology research center, baqiyatallah university of medical sciences, tehran, ir iran

conclusions findings of this study showed that integron can have a limited frequency in s. aureus isolated from clinical sample in tehran. results among the 200 samples, 1% of the strains (two isolates) contained the class 1 integron gene. the results of this study showed that the highest frequency of the obtained samples belonged to males and the isolates occurred mostly in individuals aged 51...

Journal: :journal of medical microbiology and infectious diseases 0
pantea jalali department of biology, science and research branch, islamic azad university, tehran, iran seyed fazlollah mousavi department of bacteriology & microbiology research center, pasteur institute of iran, tehran, iran nima rezaei research center for immunodeficiencies, children's medical center, tehran university of medical science, tehran, iran

introduction : haemophilus influenzae is a gram-negative bacterium causing a variety of respiratory infections in developing countries, especially in children. nasopharynx carriers of h. influenzae are the prominent source and transitional vectors of invasive diseases. as very limited information on h. influenzae carriage rate in iran was available, an evaluation on prevalence of this bacterium...

Journal: :مجله بین المللی علوم آزمایشگاهی 0
mohammad ataei ehsan farashahi morteza seifati nasrin ghasemi

background and aims: this research aimed to study a possible link between endometriosis and polymorphism of the progesterone receptor gene. materials and methods: the control group consisted of 86 women without endometriosis and the case group comprised 86 patients with a diagnosis of endometriosis by laparoscopy. genotypes for alu insertion polymorphisms (a1/a1, a1/a2 and a2/a2) were described...

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