An understanding of how the nuclear pore complex (NPC) mediates nucleocytoplasmic exchange requires a comprehensive inventory of the molecular components of the NPC and a knowledge of how each component contributes to the overall structure of this large molecular translocation machine. Therefore, we have taken a comprehensive approach to classify all components of the yeast NPC (nucleoporins). ...

Much of life's essential molecular machinery consists of large protein assemblies that currently pose challenges for structure determination. A prominent example is the nuclear pore complex (NPC), for which the organization of its individual components remains unknown. By combining stochastic super-resolution microscopy, to directly resolve the ringlike structure of the NPC, with single particl...

the characterizations of natural diatomite from birjand deposit (iran) were studied such as chemical compositions, specific surface area, total pore volume, pore size distribution and xrd. the diatomite that was treated only with sulfuric acid displayed a larger surface area, expanded total pore volume and increased pore size in comparison with both washed natural diatomite with deionized water...

Pore pressureis defined as the pressure of the fluid inside the pore space of the formation, which is also known as the formation pressure. When the pore pressure is higher than hydrostatic pressure, it is referred to as overpressure. Knowledge of this pressure is essential for cost-effective drilling, safe well planning, and efficient reservoir modeling. The main objective of this study is to ...

The 70SiO2-15TiO2-15ZrO2 membrane was prepared by a sol-gel procedure. The corrosion behavior of microporous toplayers along with the membrane characterization in terms of pore size, surface area, pore volume and weight loss is described. The final ceramic membrane with a thickness of 400 nm and uniform surface was obtained. This membrane confirmed the fine microporous characteristic with mean ...

in ziegler-natta olefin polymerization, the pore architecture of catalysts plays a crucial role in catalyticperformances and polymer properties. while the type of preparation routes (such as chemical reaction andsolution precipitation) greatly affects the catalyst pore architecture as a result of different solidification mechanisms,the modification of the pore architecture within a given route ...

Caspases were recently implicated in the functional impairment of the nuclear pore complex during apoptosis, affecting its dual activity as nucleocytoplasmic transport channel and permeability barrier. Concurrently, electron microscopic data indicated that nuclear pore morphology is not overtly altered in apoptotic cells, raising the question of how caspases may deactivate nuclear pore function...

Nuclear envelope (NE) formation during cell division in multicellular organisms is a central yet poorly understood biological process. We report that the conserved nucleoporin Nup155 has an essential function in NE formation in Caenorhabditis elegans embryos and in Xenopus laevis egg extracts. In vivo depletion of Nup155 led to failure of nuclear lamina formation and defects in chromosome segre...

Nuclear pore complexes (NPCs) are 'supramolecular complexes' on the nuclear envelope assembled from multiple copies of approximately 30 different proteins called nucleoporins (Nups) that provide aqueous channels for nucleocytoplasmic transport during interphase. Although the structural aspects of NPCs have been characterized in detail, NPC formation and its regulation, especially during interph...

Nuclear pore complexes (NPCs) are indispensable for cell function and are at the center of several human diseases. NPCs provide access to the nucleus and regulate the transport of proteins and RNA across the nuclear envelope. They are aqueous channels generated from a complex network of evolutionarily conserved proteins known as nucleporins. In this Cell Science at a Glance article and the acco...