Correct positioning of the division plane is a prerequisite for the generation of daughter cells with a normal chromosome complement. Here, we present a mechanism that coordinates assembly and placement of the FtsZ cytokinetic ring with bipolar localization of the newly duplicated chromosomal origins in Caulobacter. After replication of the polarly located origin region, one copy moves rapidly ...

Asymmetric divisions are crucial for generating cell diversity; they rely on coupling between polarity cues and spindle positioning, but how this coupling is achieved is poorly understood. In one-cell stage Caenorhabditis elegans embryos, polarity cues set by the PAR proteins mediate asymmetric spindle positioning by governing an imbalance of net pulling forces acting on spindle poles. We found...

Regulation of centrosome and spindle positioning is crucial for spatial cell division control. The one-cell Caenorhabditis elegans embryo has provenattractive for dissecting themechanismsunderlyingcentrosome and spindle positioning in a metazoan organism. Previous work revealed that these processes rely on anevolutionarily conserved force generator complex located at the cell cortex. This compl...

Division site positioning is critical for both symmetric and asymmetric cell divisions. In many organisms, positive and negative signals cooperate to position the contractile actin ring for cytokinesis. In rod-shaped fission yeast Schizosaccharomyces pombe cells, division at midcell is achieved through positive Mid1/anillin-dependent signaling emanating from the central nucleus and negative sig...

Recently the superior performance of the PolyMAX modal parameter estimation method using frequency response functions was demonstrated. The method can be implemented in a very similar way as the industry standard polyreference (time-domain) least-squares complex exponential method: in a first step a stabilisation diagram is constructed containing frequency, damping and participation information...

The mitochondrial network fragments and becomes uniformly dispersed within the cytoplasm when mammalian cells enter mitosis. Such morphology and distribution of mitochondria was previously thought to facilitate the stochastic inheritance of mitochondria by daughter cells. In contrast, we recently reported that mitochondria in dividing mammalian cells are inherited by an ordered mechanism of inh...

We present evidence that, in Escherichia coli, polar positional information is present at midcell independent of known cell division factors. In filamented cells, IcsA, which is normally polar, localizes at or near potential cell division sites. Because the cell pole is derived from the septum, the sites to which IcsA localizes in filaments correspond to future poles. IcsA localization to these...

This article reviews the Business Process Reengineering (BPR) vision of radical business process change, focusing upon the use of information technology, to facilitate a shift away from linear work organization towards parallel processing and multidisciplinary team working. To approach such a change, by focusing on the technologies aspect to the detriment of a regard on managerial, technologica...