We have developed a novel and reproducible system for recovery of fertile transgenic maize (Zea mays L.) plants. The transformation was performed using microprojectile bombardment of cultured shoot apices of maize with a plasmid carrying two linked genes, the Streptomyces hygroscopicus phosphinothricin acetyltransferase gene (bar) and the potato proteinase inhibitor II gene, either alone or in ...

Rhizosphere engineering with beneficial plant growth promoting bacteria offers great promise for sustainable crop yield. Potato is an important food commodity that needs large inputs of nitrogen and phosphorus fertilizers. To overcome high fertilizer demand (especially nitrogen), five bacteria, i.e., Azospirillum sp. TN10, Agrobacterium sp. TN14, Pseudomonas sp. TN36, Enterobacter sp. TN38 and ...

In order to enhance the system of potato transformation and further regeneration, four potato cultivars were transformed using the Agrobacterium tumefaciens harboring â-glucuronidase (GUS) gene.The results revealed that the highest percentages of shoot formation obtained were for Spunta cv. Stem explants resulted in more callus and regeneration after transformation and selection than leaf expla...

Brassinosteroids (BRs) are steroidal phytohormones that regulate various physiological processes, such as root development and stress tolerance. In the present study, we showed that brassinolide (BL) affects potato root in vitro growth in a dose-dependent manner. Low BL concentrations (0.1 and 0.01 μg/L) promoted root elongation and lateral root development, whereas high BL concentrations (1-10...

The experiment was conducted during the period from January to December, 2008 at the Laboratory of Biotechnology, Bangladesh Agricultural Research Institute, Gazipur, Bangladesh. A two step procedure was followed for direct plant regeneration of potato. Murashige and Skoog (MS) basal medium supplemented with 0.5 mg l -1 IBA and 30 g sugar was used for in vitro potato plants development via noda...

Better knowledge on responses to dehydration stress could help to improve the existing cryopreservation protocols for potato, since plant tissues processed for cryopreservation are often submitted to similar in vitro stress conditions. Cryopreservation (the best method of conservation for vegetatively propagated plants) of potato still needs to be standardized to make it available and to conser...

potato is one of the most productive crops in agriculture and is a valuable food source in developing countries. mycorrhizal fungi involve a symbiosis with most of crop roots and leads to improve the crops growth and yield. in order to investigate the effect of mycorrhiza fungi species on yield of potato cultivars a factorial experiment was arranged in a randomized complete block design with ei...

aeroponic is a new technology for potato minituber production. in order to recognition of the suitable nutrient solution and potato variety for minituber production in aeroponics in comparison with classic hydroponics, a factorial experiment was carried out with three factors of culture systems (aeroponics and hydroponics), nutrient solutions (chang et al., 2008; apcoab and commercial nutrient ...