Insertion of reporter genes into plant virus genomes is a common experimental strategy to research many aspects of the viral infection dynamics. Their numerous advantages make fluorescent proteins the markers of choice in most studies. However, the use of fluorescent proteins still has some limitations, such as the need of specialized material and facilities to detect the fluorescence. Here, we...

DNA complementary to the 3'-terminal 3684 nucleotides of the Ornithogalum mosaic potyvirus (OMV) genome was cloned and sequenced. The sequence consisted of a single large open reading frame which probably starts upstream of the cloned region. By comparison to other sequenced potyviruses, it was estimated that the clone contained the 3' non-coding (3'-NC) region, the coat protein (CP) gene and t...

Abstract Coat protein (CP) is required for potyviruses to move and establish a systemic infection in plants. π-stackings formed by aromatic residues play critical roles maintaining stability functions. As we know, many located the core region of potyvirus CPs are conserved. However, their remain largely unknown. Here, through analysis three-dimensional model tobacco vein banding mosaic virus (T...

Potyviruses constitute the second largest genus of plant viruses and cause important economic losses in a large variety of crops; however, the atomic structure of their particles remains unknown. Infective potyvirus virions are long flexuous filaments where coat protein (CP) subunits assemble in helical mode bound to a monopartite positive-sense single-stranded RNA [(+)ssRNA] genome. We present...

A vector derived from an original infectious clone of Turnip mosaic potyvirus (TuMV) has been developed. The vector (p35Tunos-vec01) was made through the creation of a new unique insertion site for foreign genes between the NIb and the CP genes of the viral genome. The jellyfish Green Fluorescent Protein (GFP) and E. coli β-glucuronidase (GUS) were expressed in Arabidopsis thaliana plants infec...

Verbena x hybrida is an ornamental annual used in rock gardens, as an edging plant, and in hanging baskets. It comes in a variety of colors and grows approximately 6 to 10 inches tall. Verbena 'Lavender Shades' plants from California showing leaf mosaic symptoms tested positive for potyvirus in an antigen-coated plate ELISA using our potyvirus broad spectrum reacting PTY-1 monoclonal as the det...

The chilli ring spot virus (ChiRSV) is one of the most important crop in Southeast Asia and China. Chilli devastating not only for but also solanaceous crops. In severe cases, up to 100% yield losses have been observed crop. A large vector population spreads this disease throughout diversity Potyvirus proven after examination many samples isolated from different locations China Vietnam, which s...

Genome-linked protein VPg of Potato virus A (PVA; genus Potyvirus) has essential functions in all critical steps of PVA infection, i.e. replication, movement, and virulence. Structural features of the recombinant PVA VPg were investigated with the aim to create an outline for structure-function relationships. Circular dichroism data of PVA VPg revealed a distinct near-UV spectrum indicating tha...

potato virus y (pvy) is one of the most important pathogenic agents that reduce the yield and quality of tobacco (nicotiana tabacum l.). dna-based molecular markers are valuable tools for identifying germplasm in gene banks, assessing kinship ties, and doing marker-assisted selection (mas) for resistance to pests, diseases, and some crop traits of interest. this work presents the results of ass...