transgene integration and expression in host plant is quite unpredictable and is considered as the major problem in plant transformation. the variation in transgene copy number in transgenic plants influences the expression level and is one of such complication. in many plant species, the analysis of transgenic plants has shown that independent transgenic plants have one to many copies of trans...

Softwarization of Network Functions (NFs) accelerates automated deployment and management services on next-gen networks. Combining flexibility high-performance is a vital requirement for Virtualisation (NFV); however, many studies have demonstrated that containerization or virtualization NFs severely degrades the fundamental efficiency packet forwarding. Virtual network I/O, mechanism transferr...

in previous years, identification of fetal cells in maternal blood circulation has caused a new revolution in non-invasive method of prenatal diagnosis. low number of fetal cells in maternal blood and long-term survival after pregnancy limited the use of fetal cells in diagnostic and clinical applications. with the discovery of cell-free fetal dna (cffdna) in plasma of pregnant women, access to...

PURPOSE To investigate the molecular mechanism relating phenotype and genotype in the rd mouse, mRNA and pre-mRNA levels derived from the wild-type and position-347 nonsense mutant beta-phosphodiesterase (beta-PDE) genes were determined and compared with the corresponding gene copy ratios. METHODS Total RNA and genomic DNA was isolated from the retinas of three heterozygous rd/+ mouse strains...

Design a clocked synchronous state machine with four inputs, G1–G4, that are connected to pushbuttons. The machine has four outputs, L1–L4, connected to lamps or LEDs located near the like-numbered pushbuttons. There is also an ERR output connected to a red lamp. In normal operation, the L1–L4 outputs display a 1-out-of-4 pattern. At each clock tick, the pattern is rotated by one position; the ...

One of the techniques for increasing flexibility and scalability of cloud data centers is Task Migration. The act of migration is performed for different goals like balancing and load sharing, energy management and reducing response time and improvement of Quality of Service. In this paper, different kinds of migrations like Pre-Copy, Post-Copy, TPM triple migration, and related issues are stud...

BACKGROUND Mitochondria are eukaryotic organelles responsible for energy production. Quantitative changes in human mitochondrial DNA (mtDNA) copy number have been implicated in various cancer types. Data from prospective cohort studies on mtDNA copy number and colorectal cancer risk have been lacking. METHODS We evaluated the association between mtDNA copy number in peripheral blood and color...

Few biomarkers exist to predict radiotherapy response in breast cancer. In vitro studies suggest a role for Met and its ligand HGF. To study this suggested role, MET and HGF gene copy numbers were determined by droplet digital PCR in tumours from 205 pre-menopausal and 184 post-menopausal patients, both cohorts randomised to receive either chemo- or radiotherapy. MET amplification was found in ...