Nutritional relationships were revealed during the coculturing of Bacteroides gingivalis with Wolinella recta and Bacteroides melaninogenicus with W. recta. W. recta produced a substance that stimulated the growth of B. gingivalis and B. melaninogenicus. Characterization by thin-layer chromatography and absorption spectrometry identified the compound as protoheme. Production of large amounts of...

Infectivity of Bacteroides asaccharolyticus (formerly B. melaninogenicus subsp. asaccharolyticus; see S. M. Finegold and E. M. Barnes, Int. J. Syst. Bacteriol. 27:388--391, 1977) was dependent on the presence of a second organism. An infective consortium consisting of B. asaccharolyticus and Klebsiella pneumoniae was defined. Neither organism was infective alone, but the Klebsiella could be rep...

Dental plaque known to harbour Bacteroides melaninogenicus was removed from the gingival half of selected buccal tooth surfaces. The plaque was dispersed, diluted and cultured on medium containing 0 per cent, 0*05,0* 1 and 0.5 per cent Kanamycin. When the medium contained 0.05 per cent Kanamycin, only B. melaninogenicus were found and the number recovered was about 20 per cent of the B. melanin...

0 N E member of the genus Bacteroides-Bacteroides melaninogenicus-characteristically produces a jet-black pigment when grown on blood agar. This property was described by Oliver and Wherry (1921) in the original account of the organism, and has since been regarded as highly distinctive. The work described in this paper is based on the chance observation that other members of the genus are also ...

was found to be an additional growth factor in that this compound can replace the requirement for heme in the presence of vitamin K, allowing good growth of the organism, and succinate can also partially replace the requirement for vitamin K in the presence of heme. The addition of succinate to a medium supplemented with both vitamin K and heme increases the growth rate of the culture. This abi...

In a series of simulated blood culture experiments, small inocula of eight different strains of Bacteroides and five strains of anaerobic cocci were added to Difco Thiol broth and Southern Group Brewer's thioglycollate. Both methods enabled all of the strains to be isolated after one to three days' incubation, with the exception of Bacteroides melaninogenicus, and most strains to survive after ...

The in vitro activities of extracts of Nigerian chewing sticks against Bacteroides gingivalis and B. melaninogenicus are presented. The greatest inhibitory action was produced by Serindeia werneckei, whereas Fagara zanthoxyloides produced no appreciable inhibitory effect. A generally good correlation was found between the killing curves and MICs. Only extracts of Anogeissus leiocarpus showed ac...

Strains of Bacteroides asaccharolyticus and Bacteroides melaninogenicus subspecies isolated from human and animal sources were examined for the production of phenylacetic acid. B. asaccharolyticus strains isolated from sites in humans and monkeys always produced phenylacetic acid. B. asaccharolyticus strains isolated from human nonoral sites consistently failed to produce this product. This met...

The plaque flora isolated from discrete dentogingival sites during a human gingivitis experiment was analyzed as a function of the plaque score and of the gingivitis score. When the gingivitis score was plotted as a function of the plaque score, a nonbleeding gingivitis was associated with a proportional increase in the Actinomyces sp. at the expense of the Streptococcus sp. In particular, the ...