Using in silico methods, several putative phytohormone-responsive cis-elements in the Oryza sativa non-symbiotic haemoglobin (NSHB) 1-4 and Arabidopsis thaliana NSHB1-2 promoters have been identified. An OsNSHB2 promoter::GUS reporter gene fusion shows tissue-specific expression in A. thaliana. GUS expression was observed in roots, the vasculature of young leaves, in flowers, and in the pedicel...

ToxR of Vibrio cholerae directly activates the ompU promoter, but requires a second activator, TcpP to activate the toxT promoter. ompU encodes a porin, while toxT encodes the transcription factor, ToxT, which activates V. cholerae virulence genes including cholera toxin and the toxin co-regulated pilus. Using an ompU-sacB transcriptional fusion, toxR mutant alleles were identified that encode ...

LOX-1, a receptor for ox-LDL (oxidized low-density lipoprotein), has recently been determined to play a critical role in the progression of atherosclerosis. LOX-1 expression (mRNA and protein) has been shown to be up-regulated by pro-atherogenic stimuli, such as ox-LDL and Ang II (angiotensin II). However, the molecular mechanisms of these up-regulations are unclear. In the present study, we ex...

Leydig cells produce androgens which are essential for male sex differentiation and reproductive functions. Steroidogenesis, as well expression of several genes in cells, stimulated by LH/cAMP repressed AMP/AMPK. One those is Plau, codes the urokinase-type plasminogen activator (uPA), a secreted serine protease. The role uPA regulation Plau remain unknown. Using siRNA-mediated knockdown, was re...

Transcription activation by Escherichia coli catabolite activator protein (CAP) at each of two classes of simple CAP-dependent promoters is understood in structural and mechanistic detail. At class I CAP-dependent promoters, CAP activates transcription from a DNA site located upstream of the DNA site for RNA polymerase holoenzyme (RNAP); at these promoters, transcription activation involves pro...

The oxidative dehydrogenation of propane with CO2 (CO2-ODP) is a promising technology for the efficient production propene in tandem reduction to CO. However, rational design high-performance catalysts this green process still challenged by limited understanding nature active sites and reaction mechanism. In work, effects SnO2 promoter on Pt/CeO2 activity selectivity CO2-ODP are elucidated thro...