Background: It was previously shown that the activity of a serine protease from a moderately halotolerant Bacillus aquimaris VITP4 strain is active in a wide range of pH and temperatures and could be modulated by the presence of the divalent metal ions.  Objectives: In the present study, a quantitative analysis was done in order to explore the parameters that are contributing to the protease a...

The production of protease by Bacillus licheniformis BBRC 100053 was studied. The most appropriate medium for the growth and protease production is composed of: lactose 1%, yeast extract 0.5%, peptone 0.5%, KH2PO4 0.1%, MgSO4.7H2O 0.02%. Enzyme production corresponded with growth and reached a maximums level (589 U/ml) during the stationary phase at 35°C, pH equivalent to 10 and with 150 rpm af...

The present study explored the efficacy of Bacillus spp. and protease production for biocontrol of the root-knot nematode Meloidogyne javanica in African violet media. Among 100 bacterial isolates from various soils, the highest nematode mortality was observed for treatments with isolate GM-18, which was identified as Bacillus subtilis based on cultural and morphological characteristics and 16S...

background: considering their specific adaption to marine milieus, marine actinomycetes constitute an excellent source for producing unique bioactive substances. they are also important sources of antibiotics and extracellular enzymes, geosmin, and enzymes with potent antibacterial and anticarcinogenic properties and low toxicity. the objective of this study is to isolate actinomycetes producin...

production of thermostable alkaline protease in tray bioreactor for solid state fermentation is a unique process which may lead to an economical approach for production of enzyme from agricultural waste or low cost substrate. in solid state fermentation using tray bioreactor, several agricultural residues such as rice bran, sugarcane bagasse, wheat bran, barely bran, corn meal and corn husk wer...

furin is a ca 2+ -dependent serine protease which cleaves proprotein substrates at the arg-xaa-(lys/arg)-arg    site to generate biologically active proteins. furin’s critical role in many cellular events associated with health disorders such as hiv, sars, anthrax, and influenza as well as cancer has made inhibitors of this enzyme as therapeutic targets. to this date, the most potent inhibitor ...

background and objectives: thermophilic bacteria are less studied but important group of microorganisms due to their ability to produce industrial enzymes. materials and methods: in this study, thermophilic bacteria were isolated from hot spring of tarabalo, india. a bacterium that could tolerate high temperatures was characterized by morphology, biochemistry and sequencing of its 16s rrna gene...

the present study was conducted to evaluate the effect of dietary inclusion of silver nanoparticle on general performance and digestive proteases in zebra fish. four experimental diets were designed with a concentration of 5, 20 and 40 parts per billion (ppb) nanoparticles in the diet (named s5, s20, and s40, respectively) and control group without any nanoparticles (s0). there was no significa...

Background and Purpose: Today, treatment of life-threatening fungal infections, caused by Candida species, has become a major problem. In the present study, we aimed to evaluate the antifungal susceptibility patterns of different clinical Candida isolates, determine the virulence factors in multi-drug resistant (MDR) Candida species, and assess the anti-biofilm activity of Elettaria cardamomum ...