Cysteine proteases are an important class of enzymes implicated in both developmental and defense-related programmed cell death and other biological processes in plants. Because there are dozens of cysteine proteases that are posttranslationally regulated by processing, environmental conditions, and inhibitors, new methodologies are required to study these pivotal enzymes individually. Here, we...

Using 20 strains of Pseudomonas aeruginosa isolated from patients, production of protease, elastase, and collagenase was determined by shaking culture in either complex or semisynthetic medium. No collagenase was produced by any strain of P. aeruginosa. According to their production of protease and elastase in different media, the P. aeruginosa strains were divided into three groups: the first ...

abstract bee venom is a complex mixture of proteins, peptides and low molecular components: proteins (enzymes) :( phospholipase, a2phospholipase b, hyaluronidase α – glucosidase) - , peptides (melittin, apamine mcd peptide,adolapine ,procamine a and b, protease inhibitors)- low molecular components: biogenic amines:( histamine, dopamine and noradrenalin) - pheromones: complex ethers- sugars :( ...

Enzymes are among the most important products obtained for human needs through microbial sources. A large number of industrial processes in area industrial, environmental and food technology utilize enzymes at some stage or another; Solid State Fermentation (SSF) holds tremendous potential production enzymes, especially those where crude fermented product may be used directly as enzyme source. ...

The potentiality of 23 bacterial isolates to produce alkaline protease and carboxymethyl-cellulase (CMCase) on Ficus nitida wastes was investigated. Bacillus pumillus ATCC7061 was selected as the most potent bacterial strain for the production of both enzymes. It was found that the optimum production of protease and CMCase were recorded at 30 °C, 5% Ficus nitida leaves and incubation period of ...

The enzymes are having wide applications in various industries. group of microorganisms, viz., bacteria, fungi, actinobacteria produces different enzymes, cellulase, chitinase, amylase, protease, lecithinase, lipase, gelatinase, etc. and these have immense market demand. work here describes isolation Trichoderma sp. study their colony characteristics on agar media. morphology images were also s...

Abstract Although the general mechanism for serine protease catalysis is well established, some questions still remain. For instance, two enzymes, neutrophil elastase and cathepsin G, have a lot of structural resemblances. However, degrades virulence factors, while G does not. This paper studies both enzymes computationally to probe their conformational differences. In process, methodology esta...

This study aimed to evaluate the effect of supplementing different protease enzymes on growth performance, intestinal morphology, and selected carcass traits in broilers fed diets reduced 3.5% crude protein (CP) amino acids (AA). One thousand one-day-old Ross 308 (41 g) were assigned five dietary treatments with ten replicates 20 birds each: (PC) a positive control diet formulated meet acid req...

A study was carried out to evaluate the effect of replacing maize with sorghum and supplementation enzymes, on growth laying performance chickens. Four hundred fifty 12-weeks old Lohmann Brown pullets were distributed randomly into six dietary treatments three replicates per treatment, each replicate had 25 birds uniform group weights at initial stage study. The included without enzymes (T1) (T...

thermophilic bacillus sp. gus1, isolated from  a soil  sample obtained from citrus garden, produced at least three proteases as detected by sodium dodecyl sulfate polyacrylamide gel electrophoresis (sds-page) and zymogram analysis. the enzymes were stable in the alkaline ph range (8.0-12.0), with the optimum temperature and ph range of the proteases being 70ºc and 6.0-12.0, respectively. all th...