The development, refinement and validation of in vitro digestibility assays for dietary protein and amino acids for single stomached mammals are reviewed. The general principles of in vitro digestibility assays and their limitations are discussed. In vitro protein digestibility assays must be accurate, rapid, cheap, simple, robust, adaptable and relevant to the processes of digestion, absorptio...

ABSTRACT- Ninety six 2-week-old British United Turkeys (BUT) Big 6 were assigned to 2 groups (4 replicates of 12 birds each). One basal diet and another one in which 20% canola meal replaced the corn starch in the basal diet were prepared. At 9, 16 and 21 wk of age, four birds from each pen slaughtered for digesta collection. The last two-third segment of the intestine, between the Meckel’s div...

BACKGROUND The FAO has recommended replacing the protein digestibility-corrected amino acid score (PDCAAS) with the digestible indispensable amino acid score (DIAAS). OBJECTIVE The objective of this study was to compare aspects underlying the calculation of the DIAAS and PDCAAS, including 1) fecal digestibility vs. ileal digestibility, 2) using a single nitrogen digestibility value for all am...

Experiments were conducted to evaluate the nutritional value of yellow-seeded rapeseed meal (YRSM). In the first experiment nutrient retention was recorded by 48 Arbor Acres-broiler chickens (28-d old) to determine AMEn (nitrogen-corrected apparent metabolizable energy), coefficient of apparent protein digestibility based on ileal digesta nitrogen, excreta nitrogen and uric acid nitrogen. The s...

To elucidate whether the digestibility is responsible for the hypocholesterolemic action of rice protein, the effects of rice proteins extracted by alkali (RP-A) and α-amylase (RP-E) on cholesterol metabolism were investigated in 7-week-old male Wistar rats fed cholesterol-free diets for 3 weeks. The in vitro and in vivo digestibility was significantly reduced by RP-A and RP-E as compared to ca...

persian sturgeon (acipenser persicus) is a valuable fish caught in the caspian sea. in this study, effect of frying, the most common cooking methods on nutritional properties of the persian sturgeon fillet were investigated. the raw and fried samples were analyzed for amino acid composition, protein and fat content, in vitro digestibility and nutritional parameters. the results showed that the ...

Five Yorkshire x Lacombe barrows (45 kg initial wt) fitted with duodenal cannulas approximately 10 cm from the pyloric sphincter were used to determine the influence of the pH of the predigestion solution (pH 1.0, 1.5, 2.0 or 2.5), pepsin activity (189 vs 377 IU/liter) and duration of predigestion (0, 1.5, 2.5 or 4.0 h) on the apparent digestibility of the protein in soybean meal (SBM), meal an...

This study investigated the possibility of using cecectomized laying hens for the determination of amino acid (AA) digestibility of protein sources as an alternative to measurements made at the terminal ileum. Toasted soybeans and corn gluten meal were used as test protein sources. A low-protein basal diet was based mainly on corn, wheat gluten, and cornstarch. In 4 other diets toasted soybeans...