The advances in therapeutics based on biological macromolecules and their pharmaceutical market have stimulated the development of separation purification techniques like ion-exchange chromatography (IEC). In this regard, effectiveness therapies depends high-purity protein fractions. Although, there are a few differences each batch due to amplification method or degradation effect, which cause ...

Bacillus thuringiensis (Bt) has been extensively used in biopesticidal formulations due to its safe environmental and human health records. The widespread use of Bt is often challenged by production as well as formulation costs which are in direct contact with downstream processing, i.e. the separation/purification step. Downstream separation/purification efficacy governs the marketabi...

background toxoplasma gondii is an obligatory intracellular protozoan parasite, which infects human beings. since the current antigens used for diagnosis or vaccination are contaminated with non parasitic material in which the parasite is grown, it is tried to produce recombinant antigens to design vaccines against toxoplasmosis, or make diagnostic kits. choosing the type of antigen to produce ...

Novel magnetic biochar has been successfully synthesized by using microwave technique, using discarded materials such as Empty Fruit Bunch (EFB). The optimized conditions for the best novel magnetic biochar synthesis are at 900 w reaction power, 20 min reaction time, and impregnation ratio 0.5 (biomas:FeCl3) The details physical and chemical analyses of novel magnetic biochar wer...

in this study, response surface methodology was used to determine the optimum raw sugar beet juice purification process conditions using bentonite to produce inverted liquid sugar. for this purpose, impact of factors on the purification process such as bentonite concentration (1-5 gr/li), ph (3.5-6) and temperature (35-95 ˚c) was investigated. for each response, a second-order polynomial model ...

The histone-like protein HU is the most-abundant DNA-binding protein in bacteria. The HU protein non-specifically binds and bends DNA as a hetero- or homodimer, and can participate in DNA supercoiling and DNA condensation. It also takes part in DNA functions such as replication, recombination, and repair. HU does not recognize any specific sequences but shows a certain degree of specificity to ...

The safety of the cells used for Advanced Therapy Medicinal Products is crucial patients. Reliable methods cell purification are very important commercialization those new therapies. With large production scale envisioned commercialization, isolation need to be efficient, robust, operationally simple and generic while ensuring biological functionality safety. In this study, we high magnetized m...

background: it is believed that bovine neutrophils contain several peptides and protein which exhibit antimicrobial activity against microorganisms such as fungi and bacteria. objectives: the purpose of this study was to isolate and purify a potential antibacterial protein from bovine neutrophil and test its anti bacterial activity. methods: neutrophils were isolated from bovine blood using dex...

background one disadvantage of expressing heterologous proteins in escherichia coli is that the proteins are frequently expressed as insoluble inclusion bodies. to avoid this problem, heterologous proteins are typically expressed as a fusion protein. maltose binding protein (mbp) is one of the widely used partners for production of recombinant fusion proteins in e. coli. mbp is among the most e...