The anti-hypercholesterolemic effect of berbamine (BBM) isolated from Rhizoma Coptidis (RC) was investigated in hypercholesterolemic zebrafish model induced by high-cholesterol (HC) diet. Zebrafish embryo assay revealed no significant difference in morphology and cell death with the treatment of BBM less than 20 μg/mL. In zebrafish larvae, the fluorescently labeled cholesterol in caudal artery ...

the objective of the present study was to determine if modifications would occur due to epinephrine perfusion and simulating stressful situations (2 µg/ kg/ min, an animal)and transportation,causing change of the values of extractable protein concentrations, sds page profiles and the calpain calpastatin complex in silver rabbit longissimus muscles resulting in consequent changes in meat tendern...

The targeting of mRNAs to neuronal dendrites is an important protein sorting mechanism. Recent studies have revealed that mRNAs are transported by molecular motors. The kinesin superfamily protein KIF5 transports mRNAs such as calcium/calmodulin-dependent kinase IIalpha (CaMKIIalpha) and Arc mRNAs along microtubules in large granules containing proteins involved in RNA transport, protein synthe...

The anti-hypercholesterolemic effect of berbamine (BBM) isolated from Rhizoma Coptidis (RC) was investigated in hypercholesterolemic zebrafish model induced by high-cholesterol (HC) diet. Zebrafish embryo assay revealed no significant difference in morphology and cell death with the treatment of BBM less than 20 μg/mL. In zebrafish larvae, the fluorescently labeled cholesterol in caudal artery ...

An impressive body of evidence has been accumulated indicating that local protein synthesis is implicated in navigation of neurite extension induced by guidance cues, such as semaphorin3A (Sema3A). We found previously that a Src type tyrosine kinase Fyn and cyclin-dependent kinase 5 (Cdk5) mediate Sema3A-signaling. We also showed that Sema3A elicits axonal transport through neuropilin-1, a rece...

1. The rapid stimulation of intestinal Ca(2+) transport observed in vitamin D-deficient chicks after receiving 1,25-dihydroxycholecalciferol has necessitated a re-evaluation of the correlation hitherto observed between this stimulation and the induction of calcium-binding protein synthesis. By 1h after a dose of 125ng of 1,25-dihydroxycholecalciferol, Ca(2+) transport is increased. This is at l...

the herpes simplex virus type 1 (hsv-1) tegument protein, vp22 has been reported to have the property of intercellular transport. the previous studies have shown that following expression of a fusion protein containing vp22 it spreads to every cell in a monolayer and concentrates in the nucleus. in spite of these reports, some studies have shown that vp22 trafficking and its nucleus accumulatio...

Neuron Local synthesis Replenishment of distal synapses LTP Input specificity Synaptic protein turnover and plasticity Microtubule RER Spine apparatus Polysome RNP Kinesin Protein synthesis machinery mRNA transport Turnover and modification of the synaptic proteome The local transcriptome and the synaptic translation machinery Direct capture and synaptic tagging Synthesis (tag) Capture Stoichio...

Transport of mRNA from nucleus to cytoplasm is critical for eukaryotic gene expression; however, the mechanism of export is unknown. Selection and screening procedures have therefore been used to obtain a family of temperature-sensitive conditional mutants of Saccharomyces cerevisiae that accumulate poly(A)+ RNA in the nucleus when incubated at 37 degrees C, as judged by in situ hybridization. ...

Purified human platelet-derived growth factor (PDGF) induces an increase in amino acid uptake via system A in quiescent human diploid fibroblasts. Cells must be exposed to PDGF for 45 min to obtain maximum transport stimulation. Transport stimulation requires protein synthesis. Transient exposure to PDGF, alone, in the absence of plasma components can stimulate transport. Acid-insoluble [3H]leu...