the use of in vitro potato shoot cultures for protoplast isolation is desirable for consistent protoplast qualities. ethylene build-up in the culture vessels causes problems for leaf growth (e.g. decreasing leaf surface, dry weight and chlorophyll) in shoot culture. fifty to one hundred µm silver thiosulfate (sts) produced a larger leaf area as a source of plant material for protoplast isolatio...

Cosynthesis in mixed culture and protoplast fusion of non-producing mutants of Streptomyces hygroscopicus which may produce biosynthetic intermediates of bialaphos (AMPBA) were studied. Non-producing mutants were obtained by mutagenesis with N-methyl-N'-nitro-N-nitrosoguanidine (NTG) treatment, and six stable non-producing mutants were used for cosynthesis and protoplast fusion studies. The bio...

It was proved that the protoplasts prepared from mesophyll of Nicotiana tabacum are infected by tobacco mosaic virus. The infection occurred when purified tobacco mosaic virus particles were added to a protoplast suspension in the presence of poly-L-ornithine. The virus multiplied in these protoplasts to a level of 10(6) virus particles per infected protoplast during 24 hours of incubation. The...

Membrane phospholipid was found to be hydrolyzed presumably by an intracellular phospholipase C, and diglyceride-rich membrane vesicles were released from the cells during protoplast formation in Bacillus cereus Bacillus subtilis, Micrococcus lysodeikticus, and Staphylococcus aureus. The released membranes consisted mainly of small vesicles of 50 to 100 nm in diameter. They have a lower density...

Dibasic amino acids and polyamines added to oat (Avena sativa L.) leaf protoplast isolation media decrease the RNase activity of extracted protoplasts relative to controls. This effect, which is manifested even when the added polyamine is removed by exhaustive dialysis prior to assay, is due to a prevention of the rise in RNase activity which usually follows protoplast isolation. Polyamines, bu...

In vitro plant tissue culture and biotechnology used to assist support the development of breeding when classical methods propagation must be accelerated or it was necessary overcome barriers inaccessible by approaches. asparagus, improve multiple tasks, a high number in have been used, such as regeneration through organogenesis, embryogenesis, manipulation ploidy, protoplast isolation, genetic...

Mycobacillin partially quenched the strong fluorescence when 1-anilino naphthalene 8-sulfonate (ANS) was added to protoplast or plasma membrane but is without any effect on weak fluorescence when added to cell-free extract. There are two classes of ANS binding sites on protoplast or plasma membrane of which one class is sensitive to mycobacillin, being competitively abolished by it. Mycobacilli...

/ 11 Abstract / 12 / 13 One prerequisite for plant functional genomic projects is the development of a high-throughput transformation platform. As the / 14 moss Physcomitrella patens can be transformed via PEG-mediated DNA-uptake into protoplasts, a semi-continuous bioreactor / 15 culture of this plant was optimised regarding protoplast isolation efficiencies. Under standard conditions protopla...

The existence of beta-lectins on protoplast surfaces is confirmed by the agglutination of protoplasts by those Yariv antigens that have sugar specificities which also interact with isolated beta-lectins. Agglutination by beta-maltosyl but not by beta-D-mannosyl Yariv antigens is used to identify some of the structural features required of the ligand for beta-lectin binding. Inhibition of agglut...