Sweet cherry (Prunus avium L.) is one of the most important stone fruit species in Bulgaria. The known to be susceptible more than 30 viruses. Among them are prune dwarf virus (PDV), prunus necrotic ringspot (PNRSV) and apple mosaic (ApMV), ilarviruses. Testing for these viruses required certification schemes order provide virus-tested propagated material fruit-growing industry prevent their di...

We conducted a survey of the phytosanitary status Prunus germplasm collection in Nikita Botanical Gardens, Yalta, Russia. The virome plants displaying virus-like symptoms was studied using Illumina MiSeq high-throughput sequencing. Reads related to prunus necrotic ringspot virus (PNRSV), prune dwarf (PDV), and ourmia-like 1 (OuLV1) were generated number samples. Near complete genomes two diverg...

We previously showed that the movement protein (MP) gene of Alfalfa mosaic virus (AMV) is functionally exchangeable for the cell-to-cell transport of the corresponding genes of Tobacco mosaic virus (TMV), Brome mosaic virus, Prunus necrotic ringspot virus, Cucumber mosaic virus and Cowpea mosaic virus. We have analysed the capacity of the heterologous MPs to systemically transport the correspon...

The distribution of Ilarvirus species populations amongst 61 Australian Prunus trees was determined by next generation sequencing (NGS) of amplicons generated using a genus-based generic RT-PCR targeting a conserved region of the Ilarvirus RNA2 component that encodes the RNA dependent RNA polymerase (RdRp) gene. Presence of Ilarvirus sequences in each positive sample was further validated by Sa...