In the photosynthetic light reactions of plants and cyanobacteria, plastocyanin (Pc) plays a crucial role as an electron carrier and shuttle protein between two membrane protein complexes: cytochrome b(6)f (cyt b(6)f) and photosystem I (PSI). The rapid turnover of Pc between cyt b(6)f and PSI enables the efficient use of light energy. In the Pc-cyt b(6)f and Pc-PSI electron transfer complexes, ...

Ycf4 is a thylakoid protein essential for the accumulation of photosystem I (PSI) in Chlamydomonas reinhardtii. Here, a tandem affinity purification tagged Ycf4 was used to purify a stable Ycf4-containing complex of >1500 kD. This complex also contained the opsin-related COP2 and the PSI subunits PsaA, PsaB, PsaC, PsaD, PsaE, and PsaF, as identified by mass spectrometry (liquid chromatography-t...

In Dictyostelium discoideum strain V12M2, at a very low cell density (approximately 10(2) cells/cm2), most amoebae differentiate into prespore cells in a salt solution containing cAMP if an adequately diluted conditioned medium (CM) is provided (Oohata, A. A. (1995) Differentiation 59, 283-288). This finding suggests the presence of factor(s) released into the medium that are involved in induci...

This note pursues the techniques of modified psi classes on the stack of stable maps (cf. [Graber-Kock-Pandharipande]) to give concise solutions to the characteristic number problem of rational curves in P2 or P1×P1 with a cusp or a prescribed triple contact. The classes of such loci are computed in terms of modified psi classes, diagonal classes, and certain codimension-2 boundary classes. Via...

The aim of this research is to investigate the terrain deformation in the metropolitan area of Sydney using multiple satellite radar imagery. The persistent scatterer interferometry (PSI) strategy developed at Geodesy and Earth Observing Systems Group (GEOS) of the University of New South Wales (UNSW) is used to map the land deformation in Sydney with C-band ENVISAT radar images in this study. ...

Suppose [(i), i>or=1] and [Y(i), i>or=1] are two independent sequences with distribution functions F(X)(x) and F(Y)(x), respectively. Z(i,n) is the combination of X(i) and Y(i) with a probability p(n) for each i with 1<or=i<or=n. The extreme value distribution G(Z)(x) of this particular triangular array of the i.i.d. random variables Z(1,n), Z(2,n), ..., Z(n,n) is discussed. We found a new form...

We present fiber Bragg grating pressure sensors in air-hole microstructured fibers for high-temperature operation above 800 degrees C. An ultrafast laser was used to inscribe Type II grating in two-hole optical fibers. The fiber Bragg grating resonance wavelength shift and peak splits were studied as a function of external hydrostatic pressure from 15 psi to 2000 psi. The grating pressure senso...

The effect of the modified nucleoside pseudouridine (psi) on RNA structure was compared with uridine. The extent of base stacking in model RNA oligonucleotides was measured by 1H NMR, UV, and CD spectroscopy. The UV and CD results indicate that the model single-stranded oligoribonucleotides AAUA and AA psi A form stacked structures in solution and the CD results for AA psi A are consistent with...

Prior work by others has shown that insertion of psi (i.e., leader) sequences from the Moloney murine leukemia virus (MLV) genome into the 3' untranslated region of a nonviral mRNA leads to the specific encapsidation of this RNA in MLV particles. We now report that these RNAs are, like genomic RNAs, encapsidated as dimers. These dimers have the same thermostability as MLV genomic RNA dimers; li...