We optimized the expression and purification of outer membrane proteins SpaO LamB from Salmonella typhi. investigated various factors in processes, including use isopropyl β-d-1 thiogalactopyranoside (IPTG), imidazole, urea. First, PCR amplification was carried out on genes. The genes were then cloned pTZ57R/T, expressed pET28a vector transformed into Escherichia coli BL21 (DE3). Gene insertion...

objective: leishmaniasis is caused by parasitic protozoa of the genus leishmania which in the infected host is obligate intracellular parasite. lack gene is conserved among related leishmania species. lack is the immuno-dominant antigen of l.major which is considered as the most promising molecule for a recombinant or dna vaccine against leishmaniasis. materials and methods: in this study the ...

conclusions the results of this study demonstrated that the p450 cyp141 gene was successfully cloned into a pet26b plasmid vector as an expression vector. in this paper, for the first time in iran, this gene was cloned for more purposes, including the expression and purification of the recombinant cytochrome p450 cyp141 protein. results the cloning of p450 cyp141 gene was confirmed by the enzym...

AIM We aimed to develop a multiplex PCR assay for specific detection of EPEC and EHEC pathotypes based on specific marker genes. BACKGROUND About 2.5 million infant's morbidity in developing countries occurs by E.coli pathotypes because of diarrhea and intestinal diseases. The traditional phenotypic methods are time consuming and sometimes detection and differentiation of the pathotypes are n...

BabA2, one of the two allele of BabA gene and a member protein of Helicobacter pylori, plays a vital role in assisting bacterial colonization in the stomach. However, its association with H pylori -related gastroduodenal diseases still remains unclear. In the present study, babA2 gene from Helicobacter pylori was amplified using specific primers and then cloned in pTZ57R/T and transformed into ...

Recombinant human Bone Morphogenetic Protein 2 (rhBMP2) has important applications in the spine fusion and ortho/maxillofacial surgeries. Here we first report the secretory expression of biological active dimerized rhBMP2 from Bacillus subtilis system. The mature domain of BMP2 gene was amplified from pTz57R/BMP2 plasmid. By using pHT43 expression vector two constructs, pHT43-BMP2-M (single BMP...

BACKGROUND AND THE PURPOSE OF THE STUDY Angiogenesis is an important process in physiology and disease pathogenesis and is controlled in a healthy body by a number of stimulatory and inhibitory factors. The aim of this study was to determine the effect of antisense transcript on the sense transcript of the endothelial growth factor (EGF) gene in bacterial system as an approach for the gene regu...