HIV-1(HXB2) 5'LTR region, most of BIV(R29) gag-pol segment and HIV-1(HXB2) pol IN-3'LTR region were respectively amplified. A chimeric clone, designated as pHBIV(3753), was constructed by cloning three fragments sequentially into pUC18. MT4 cells were transfected with pHBIV3753. The replication and expressions of the chimeric virus (HBIV3753) were monitored by RT activity and IFA. The results f...

SOURCE/DESCRIPTION: A 7.5 kb Bglll fragment from the cosmid RMR6 was subcloned into the BamHI site of pUC18. L POLYMORPHISM: TaqI identifies a 2 allele polymorphism with a r band at 2.5 kb (Tl) or 2.3 kb (T2) with a constant band at 1.5 h kb. CHROMOSOMAL LOCALIZATION: This probe has been assigned to chromosome 20 by linkage analysis (1) with a locus (D2OS1) known to span this chromosome (2). • ...

We developed a counterselectable deletion system for Thermus thermophilus HB27 based on cytosine deaminase (encoded by codA) from Thermaerobacter marianensis DSM 12885 and the sensitivity of T. thermophilus HB27 to the antimetabolite 5-fluorocytosine (5-FC). The deletion vector comprises the pUC18 origin of replication, a thermostable kanamycin resistance marker functional in T. thermophilus HB...

Antimicrobial activity of Ribes nigrum L. and rubrum leaf extracts was determined by disk-diffusion method as well specific growth rate generation succeeding factor determination. Different Gram-positive (Bacillus subtilis WT-A, Staphylococcus aureus MDC 5233, Enterococcus hirae ATCC 9790) Gram-negative (Escherichia coli 25922, Salmonella typhimurium MDC1754, ampicillin resistant E. dhpα-pUC18 ...

The beta-ketoadipate pathway of Acinetobacter calcoaceticus comprises two parallel metabolic branches. One branch, mediated by six enzymes encoded by the cat genes, converts catechol to succinate and acetyl coenzyme A (acetyl-CoA); the other branch, catalyzed by products of the pca genes, converts protocatechuate to succinate and acetyl-CoA by six metabolic reactions analogous or identical to t...

Population levels of total and ampicillin-resistant culturable bacteria and the putative horizontal bla gene acquirement from Bt-corn were studied in commercial fields of transgenic corn in Spain during the years 2000-2003. Commercial fields consisting of conventional corn (Dracma) and Bt176 transgenic corn (Compa CB) were located in three climatic regions. The effect of corn type, plant materi...