The stromal pH of the chloroplast must be maintained higher than that of the surrounding cytosol for photosynthetic carbon assimilation to occur. Experimental evidence demonstrating how this is accomplished in the plant cell is lacking. In the experiments reported here, we studied H+ and K+ flux across membranes of purified chloroplast inner-envelope vesicles. We were able to demonstrate ATP-de...

In crude extracts of plant tissue, the M(r) = 100,000 proton-pumping ATPase constitutes less than 0.01% of the total cell protein. A large-scale purification procedure is described that has been used to obtain extensive protein sequence information from this enzyme. Plasma membrane vesicles enriched in ATPase activity were obtained from extracts of oat roots by routine differential and density ...

Lysophosphatidylcholine at concentrations of 30 micromolar stimulated the rate of MgATP-dependent H(+)-accumulation in oat (Avena sativa L. cv Rhiannon) root plasma membrane vesicles about 85% while the passive permeability of H(+) was unchanged. Activation was dependent on chain length, degree of saturation, and head group of the lysophospholipid. A H(+)-ATPase assay was developed that allowed...

KVWPCA operates a waste water treatment plant in Leechburg, Pennsylvania, about 40 kilometers (25 miles) northeast of Pittsburgh on the flood plain of the Kiskiminetas River. From 1976 to 1993, KVWPCA treated sewage sludge by incineration. KVWPCA disposed of the resulting sewage sludge ash by mixing it with water to form a liquid slurry and pumping this material into an onsite lagoon. Discharge...

A data-mining approach is proposed to model a pumping system in a wastewater treatment plant. Two parameters, energy consumption and wastewater flow rate after the pumping system, are used to evaluate the performance of 27 scenarios while the pump was operating. Five data-mining algorithms are applied to identify the relationships between the outputs (energy consumption and wastewater flow rate...

The Report covers the design and development of a plant for pumping a liquid through a model so as to control its temperature, the design and manufacture of the model, and the development of the heat-flow meters used in the model and the means of calibrating them. Sample measurements of heat transfer on the model, a rhombic cone of unit aspect ratio at a Mach number of 2.45, are shown and compa...

In order to implement an integrated optical quantum circuit, designing waveguides based on the quantum box is of prime importance. To do this we have investigated optical waveguide both with and without optical pumping. The rate of absorption and emission using an array of AlGaAs/GaAs quantum box core/shell structure in the optical waveguide with various pumping intensities has computed. By con...

abstract background: the pressure of consumption spots in eater supply systems is one of the most important hydraulic parameters can be used in optimized management of water distribution networks. since the pressure has some different effects on various parameters of the network management including its hydraulic functions, reliability, stability and leakage, its control is of great importance ...