Isolation and identification of key components of the crude biosurfactant produced by Lactococcus lactis 53 was studied. Fractionation was achieved by hydrophobic interaction chromatography which allowed the isolation of a fraction rich in glycoproteins. Molecular (by Fourier transform infrared spectroscopy) and elemental compositions (by X-ray photoelectron spectroscopy) were determined. Criti...

this study presents a detailed analysis of cost management for intensive cultivation activity in terms of tomato production. the main objective was to provide qualitative and quantitative costs obtained in the intensive cultivation of tomato. this methodology was structured by activity costs (soil preparation and planting, crop monitoring, harvesting, selling, etc.) through a study in greenhous...

BACKGROUND The enzyme cellobiose dehydrogenase (CDH) can be used to oxidize lactose to lactobionic acid. As Sclerotium rolfsii is known to be a good producer of CDH, the aim of this paper was to simplify its production and secondly to systematically study its purification aiming for a high yield. Two preservation methods (freezing and freeze-drying) and the influence of several protectants were...

Due to the high costs associated with purification of recombinant proteins the protocols need to be rationalized. For high-throughput efforts there is a demand for general methods that do not require target protein specific optimization . To achieve this, purification tags that genetically can be fused to the gene of interest are commonly used. The most widely used affinity handle is the hexa-h...

Pigments from microalgae and cyanobacteria have attracted great interest for industrial applications due to their bioactive potential natural product attributes. These pigments are usually sold as extracts, overcome purification costs. The extraction of these compounds is based on cell disruption methodologies chemical solubility compounds. Different been used pigment extraction, such sonicatio...

In the last decades, membrane processes have gained a significant share of the market for wastewater purification. Although the product (i.e., purified water) is not of high added value, these processes are feasible both technically and from an economic point of view, provided the flux is relatively high and that membrane fouling is strongly inhibited. By controlling membrane fouling, the membr...

The cathelicidin derived human peptide LL37 has a broad spectrum of antimicrobial and immunomodulatory activities. The large variety of biological activities makes LL37 a very promising candidate for clinical applications. The production of biologically active LL37 in large amounts with reduced costs can only be achieved using recombinant techniques. In this work, LL37 has been cloned to the N-...

We carried out the purification of C-phycocyanin and allophycocyanin from Spirulina platensis taking advantage of the adsorption properties of the expanded beds. Initially, phycobiliproteins were released from the microalga cells by osmotic shock. Next, phycocyanins were recovered by applying the centrifuged cell suspension directly to the anion exchanger Streamline-DEAE using expanded bed colu...

Chlorination, ozonation and non-thermal plasma water purification technologies were compared in terms of their effectiveness, efficiency, capital operating costs, energy yield chemical demand. Retrofitting technology to chlorination plants offered the lowest cost (ZAR 14,000 or USD 253,376 based on current South African Reserve Bank rate ZAR/USD 18.0983) most effective contaminant removal (of t...